Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)
![Western blot - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)](https://www.abcam.com/ps/products/24/ab24859/Images/ab24859-35670-anti-tie2-antibody-cl-16-western-blot.jpg "Western blot - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)")
Key features and details
- Mouse monoclonal [Cl. 16] to TIE2 - BSA and Azide free
- Suitable for: IHC-Fr, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TIE2 antibody [Cl. 16] - BSA and Azide free
See all TIE2 primary antibodies -
Description
Mouse monoclonal [Cl. 16] to TIE2 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TIE2. Recombinant human soluble extracellular TIE2.
Database link: Q02763 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Cl. 16 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 126 kDa.
|
| Flow Cyt |
Use 1-2µg for 106 cells.
We tested in-house with methanol-fixed cells, but this may not be necessary since the antibody recognies an extracellular epitope. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 126 kDa. |
|
Flow Cyt
Use 1-2µg for 106 cells. We tested in-house with methanol-fixed cells, but this may not be necessary since the antibody recognies an extracellular epitope. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1. -
Tissue specificity
Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney. -
Involvement in disease
Dominantly inherited venous malformations
May play a role in a range of diseases with a vascular component, including neovascularization of tumors, psoriasis and inflammation. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Domain
The soluble extracellular domain is functionally active in angiopoietin binding and can modulate the activity of the membrane-bound form by competing for angiopoietins. -
Post-translational
modificationsProteolytic processing leads to the shedding of the extracellular domain (soluble TIE-2 alias sTIE-2).
Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner, where Tyr-992 in the kinase activation loop is phosphorylated first, followed by autophosphorylation at Tyr-1108 and at additional tyrosine residues. ANGPT1-induced phosphorylation is impaired during hypoxia, due to increased expression of ANGPT2. Phosphorylation is important for interaction with GRB14, PIK3R1 and PTPN11. Phosphorylation at Tyr-1102 is important for interaction with SHC1, GRB2 and GRB7. Phosphorylation at Tyr-1108 is important for interaction with DOK2 and for coupling to downstream signal transduction pathways in endothelial cells. Dephosphorylated by PTPRB.
Ubiquitinated. The phosphorylated receptor is ubiquitinated and internalized, leading to its degradation. -
Cellular localization
Cell membrane. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Secreted. Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted. - Information by UniProt
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Database links
- Entrez Gene: 7010 Human
- Omim: 600221 Human
- SwissProt: Q02763 Human
- Unigene: 89640 Human
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Alternative names
- Angiopoietin 1 receptor antibody
- Angiopoietin-1 receptor antibody
- CD202b antibody
see all
Images
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Western blot - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)
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![Immunohistochemistry (Frozen sections) - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)](https://www.abcam.com/ps/products/24/ab24859/Images/ab24859-35671-anti-tie2-antibody-cl-16-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)ab24859 staining TIE2 in Human spleen by Immunohistochemistry (Frozen sections). -
![Immunohistochemistry (Frozen sections) - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)](https://www.abcam.com/ps/products/24/ab24859/Images/ab24859-35669-anti-tie2-antibody-cl-16-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of Human brain tissue, staining TIE2 with ab24859.
Tissue was fixed with paraformaldehyde, permeabilized with 0.25 Triton X-100 and blocked with 2.5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2.5% horse serum) for 18 hours at 4°C. An HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody. -
![Flow Cytometry - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)](https://www.abcam.com/ps/products/24/ab24859/Images/ab24859-35673-anti-tie2-antibody-cl-16-flow-cytometry.jpg)
Flow Cytometry - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)Overlay histogram showing JEG-3 cells stained with ab24859 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24859, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in JEG-3 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Western blot - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)](https://www.abcam.com/ps/products/24/ab24859/Images/ab24859-35672-anti-tie2-antibody-cl-16-western-blot.jpg)
Western blot - Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)All lanes : Anti-TIE2 antibody [Cl. 16] - BSA and Azide free (ab24859)
Lane 1 : HUVECs left untreated
Lane 2 : HUVECs stimulated for 3 hours with PMA at 25 ng/ml
Lane 3 : HUVECs stimulated for 6 hours with PMA at 25 ng/ml
Lane 4 : HUVECs stimulated for 9 hours with PMA at 25 ng/ml
Lane 5 : HUVECs stimulated for 24 hours with PMA at 25 ng/ml
Predicted band size: 126 kDa
Samples were immunoprecipitated with another TIE2 antibody.
Protocols
Datasheets and documents
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Datasheet download
References (19)
ab24859 has been referenced in 19 publications.
- Zhang Y et al. DPSCs treated by TGF-ß1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling. Stem Cell Res Ther 12:281 (2021). PubMed: 33971955
- Dou B et al. The roles of metastasis-related proteins in the development of giant cell tumor of bone, osteosarcoma and Ewing's sarcoma. Technol Health Care 29:91-101 (2021). PubMed: 33682749
- Peng T et al. miR-19a-3p Facilitates Lung Adenocarcinoma Cell Phenotypes by Inhibiting TEK. Cancer Biother Radiopharm N/A:N/A (2021). PubMed: 33493418
- Shi C et al. NEAT1 promotes the repair of abdominal aortic aneurysms of endothelial progenitor cells via regulating miR-204-5p/Ang-1. Am J Transl Res 13:2111-2126 (2021). PubMed: 34017378
- Koch PD et al. Imaging of Tie2 with a Fluorescently Labeled Small Molecule Affinity Ligand. ACS Chem Biol 15:151-157 (2020). PubMed: 31809013
