Recombinant Anti-TIMP1 antibody [EPR18352] (ab211926)
![Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-388960-anti-timp1-antibody-epr18352-western-blot-human-knockout-lysate.jpg "Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18352] to TIMP1
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TIMP1 antibody [EPR18352]
See all TIMP1 primary antibodies -
Description
Rabbit monoclonal [EPR18352] to TIMP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human prostate cancer lysate; HeLa, SK-OV-3 and HT-29 whole cell lysates. IHC-P: Human colon, pancreas, lung cancer, medullary thyroid carcinoma and prostate cancer tissues. ICC/IF: SK-OV-3 and HT-29 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18352 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| WB | (1) |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
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| ICC/IF | (3) |
1/500.
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| Notes |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). |
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ICC/IF
1/500. |
Target
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
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Alternative names
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
Images
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Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)All lanes : Anti-TIMP1 antibody [EPR18352] (ab211926) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TIMP1 knockout HeLa cell lysate
Lane 3 : HT-1080 treated with 200ng/ml 12-O Tetradecanoylphorbol-13-acetate (TPA) for 24 hours, cell lysate
Lane 4 : Untreated HT-1080 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab211926 observed at 26 kDa. Red - loading control ab7291 observed at 50 kDa.
ab211926 Anti-TIMP1 antibody [EPR18352] was shown to specifically react with TIMP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261740 (knockout cell lysate ab257291) was used. Wild-type and TIMP1 knockout samples were subjected to SDS-PAGE. ab211926 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262152-anti-timp1-antibody-epr18352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human lung cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262158-anti-timp1-antibody-epr18352-western-blot.jpg)
Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)Anti-TIMP1 antibody [EPR18352] (ab211926) at 1/1000 dilution + Human prostate cancer lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16517973).
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![Immunocytochemistry/ Immunofluorescence - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262147-anti-timp1-antibody-epr18352-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TIMP1 antibody [EPR18352] (ab211926)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling TIMP1 with ab211926 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SK-OV-3 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab211926 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262156-anti-timp1-antibody-epr18352-western-blot.jpg)
Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)All lanes : Anti-TIMP1 antibody [EPR18352] (ab211926) at 1/1000 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Binding in these cell lines was extremely weak and requires optimization.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262155-anti-timp1-antibody-epr18352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human colon neuroendocrine cell is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262144-anti-timp1-antibody-epr18352-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TIMP1 antibody [EPR18352] (ab211926)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling TIMP1 with ab211926 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab211926 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262154-anti-timp1-antibody-epr18352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human islet is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262151-anti-timp1-antibody-epr18352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human medullary thyroid carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-262149-anti-timp1-antibody-epr18352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human prostate cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-TIMP1 antibody [EPR18352] (ab211926)](https://www.abcam.com/ps/products/211/ab211926/Images/ab211926-11-benefits-of-recombinant-antibodies.png)
Anti-TIMP1 antibody [EPR18352] (ab211926)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (20)
ab211926 has been referenced in 20 publications.
- Shen H et al. Curcumin analog B14 has high bioavailability and enhances the effect of anti-breast cancer cells in vitro and in vivo. Cancer Sci 112:815-827 (2021). PubMed: 33316116
- Yang Y & Min Z Effect of long non-coding RNA AK021443 on promoting hepatic fibrosis in vitro. Mol Med Rep 23:N/A (2021). PubMed: 33495828
- Li W et al. HBV induces liver fibrosis via the TGF-ß1/miR-21-5p pathway. Exp Ther Med 21:169 (2021). PubMed: 33456536
- Liu Z et al. Downregulation of miR-29b-3p promotes a-tubulin deacetylation by targeting the interaction of matrix metalloproteinase-9 with integrin ß1 in nasal polyps. Int J Mol Med 48:N/A (2021). PubMed: 33982786
- Liu Y et al. Long non-coding RNA HULC regulates TLR4 expression by acting as ceRNA to attract miR-663b in skin fibroblasts of pediatric burns. Am J Transl Res 13:2499-2510 (2021). PubMed: 34017408