Recombinant Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)](https://www.abcam.com/ps/products/155/ab155992/Images/ab155992-4-anti-tip-1-antibody-epr7900-2-immunohistochemistry-thyroid-carcinoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7900(2)] to TIP-1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TIP-1 antibody [EPR7900(2)]
See all TIP-1 primary antibodies -
Description
Rabbit monoclonal [EPR7900(2)] to TIP-1 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TIP-1 aa 1-100. The exact sequence is proprietary.
Database link: O14907 -
Positive control
- WB: Mouse lung and heart tissue lysates. Rat lung and heart tissue lysate. NIH/3T3, A673, LLC1, and C6 whole cell lysates; IHC-P: Human thyroid carcinoma tissue; Flow-cyt: A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7900(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
For unpurified use at 1/100 - 1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
1/1000. Predicted molecular weight: 14 kDa.
For unpurified use at 1/1000 - 1/10000. |
| IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| Notes |
|---|
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Flow Cyt (Intra)
1/200. For unpurified use at 1/100 - 1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000. Predicted molecular weight: 14 kDa. For unpurified use at 1/1000 - 1/10000. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
May play a role in the Rho signaling pathway. May act as an inhibitor of the Wnt signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6. -
Tissue specificity
Detected in various cell lines including HeLa. Weakly expressed in peripheral blood leukocytes. -
Sequence similarities
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 30851 Human
- Entrez Gene: 76281 Mouse
- Entrez Gene: 360564 Rat
- SwissProt: O14907 Human
- SwissProt: Q9DBG9 Mouse
- SwissProt: Q4QQV1 Rat
- Unigene: 12956 Human
- Unigene: 371656 Mouse
see all -
Alternative names
- Glutaminase interacting protein 3 antibody
- Glutaminase-interacting protein 3 antibody
- Tax interaction protein 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TIP-1 with purified ab155992 at 1/1000 dilution (2.05 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)](https://www.abcam.com/ps/products/155/ab155992/Images/ab155992-392502-anti-tip-1-antibody-epr7900-2-westernblot-human-mouse-rat.jpg)
Western blot - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)All lanes : Anti-TIP-1 antibody [EPR7900(2)] (ab155992) at 1/1000 dilution (Purified)
Lane 1 : A-673 (Human muscle Ewing's Sarcoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : LLC1 (Mus musculus lung rounded-loosely attached or floating) whole cell lysate
Lane 4 : Mouse lung lysate
Lane 5 : Mouse heart lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 7 : Rat lung lysate
Lane 8 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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![Flow Cytometry (Intracellular) - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)](https://www.abcam.com/ps/products/155/ab155992/Images/ab155992-5-anti-tip-1-antibody-epr7900-2-flowcytometry-a-673-human.jpg)
Flow Cytometry (Intracellular) - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)Intracellular Flow Cytometry analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling TIP-1 with purified ab155992 at 1/200 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Anti-TIP-1 antibody [EPR7900(2)] (ab155992)](https://www.abcam.com/ps/products/155/ab155992/Images/ab155992-6-benefits-of-recombinant-antibodies.png)
Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155992 has not yet been referenced specifically in any publications.