Recombinant Anti-TIP30 antibody [EPR13179] (ab177961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13179] to TIP30
- Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TIP30 antibody [EPR13179]
See all TIP30 primary antibodies -
Description
Rabbit monoclonal [EPR13179] to TIP30 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TIP30 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BUP3 -
Positive control
- HeLa, A431, and fetal liver lysates; Human pancreas and placenta tissues; A431 cells; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13179 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/10 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
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ICC/IF |
1/50 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Function
Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect. -
Tissue specificity
Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas. -
Domain
Unique C-terminus confers high proteasome-dependent instability to isoform 2. -
Cellular localization
Cytoplasm. Nucleus envelope. - Information by UniProt
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Database links
- Entrez Gene: 10553 Human
- Entrez Gene: 53415 Mouse
- Entrez Gene: 292935 Rat
- Omim: 605628 Human
- SwissProt: Q9BUP3 Human
- SwissProt: Q9Z2G9 Mouse
- Unigene: 90753 Human
- Unigene: 20801 Mouse
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Alternative names
- 30 kDa HIV 1 TAT interacting protein antibody
- 30 kDa HIV-1 TAT-interacting protein antibody
- 30 kDa HIV1 TAT interacting protein antibody
see all
Images
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All lanes : Anti-TIP30 antibody [EPR13179] (ab177961) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TIP30 using ab177961 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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Immunofluorescence analysis of A431 cells labeling TIP30 using ab177961 at 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
Immunohistochemical analysis of Human placenta tissue labeling TIP30 using ab177961 at 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
Immunohistochemical analysis of Human pancreas tissue labeling TIP30 using ab177961 at 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab177961 at 1/10 dilution to immunoprecipitate TIP30, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab177961 has been referenced in 4 publications.
- Lin X et al. Long Non-Coding RNA NONHSAT076754 Promotes Invasion and Metastasis in Epithelial Ovarian Cancer. J Cancer 10:1930-1940 (2019). PubMed: 31205552
- Grund A et al. TIP30 counteracts cardiac hypertrophy and failure by inhibiting translational elongation. EMBO Mol Med 11:e10018 (2019). PubMed: 31468715
- Yang Q et al. Metformin Enhances the Effect of Regorafenib and Inhibits Recurrence and Metastasis of Hepatic Carcinoma After Liver Resection via Regulating Expression of Hypoxia Inducible Factors 2a (HIF-2a) and 30 kDa HIV Tat-Interacting Protein (TIP30). Med Sci Monit 24:2225-2234 (2018). PubMed: 29654226
- You A et al. Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models. J Hematol Oncol 9:20 (2016). WB ; Human . PubMed: 26957312