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RecombinantRabMAb

Recombinant Anti-TIP30 antibody [EPR13179] (ab177961)

Submit a review Submit a question References (4)
Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Anti-TIP30 antibody [EPR13179] (ab177961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13179] to TIP30
  • Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TIP30 antibody [EPR13179]
    See all TIP30 primary antibodies

  • Description

    Rabbit monoclonal [EPR13179] to TIP30

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TIP30 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BUP3

  • Positive control

    • HeLa, A431, and fetal liver lysates; Human pancreas and placenta tissues; A431 cells; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
ICC/IF
1/50 - 1/100.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
ICC/IF
1/50 - 1/100.

Target

  • Function

    Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

  • Tissue specificity

    Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.

  • Domain

    Unique C-terminus confers high proteasome-dependent instability to isoform 2.

  • Cellular localization

    Cytoplasm. Nucleus envelope.
  • Information by UniProt

  • Database links

    • Alternative names

      • 30 kDa HIV 1 TAT interacting protein antibody
      • 30 kDa HIV-1 TAT-interacting protein antibody
      • 30 kDa HIV1 TAT interacting protein antibody
      • CC3 antibody
      • FLJ26963 antibody
      • HIV 1 Tat interacting protein 2 30kDa antibody
      • HIV 1 Tat interacting protein 2 antibody
      • HIV-1 TAT-interactive protein 2 antibody
      • HTAI2_HUMAN antibody
      • HTATIP 2 antibody
      • HTATIP2 antibody
      • Oxidoreductase HTATIP2 antibody
      • SDR44U1 antibody
      • Short chain dehydrogenase/reductase family 44U member 1 antibody
      • Tat interacting protein (30kDa) antibody
      see all

    Images

    • Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
      Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
      All lanes : Anti-TIP30 antibody [EPR13179] (ab177961) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 27 kDa

    • Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13179] (ab177961)
      Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13179] (ab177961)

      Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TIP30 using ab177961 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)

      Immunofluorescence analysis of A431 cells labeling TIP30 using ab177961 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)

      Immunohistochemical analysis of Human placenta tissue labeling TIP30 using ab177961 at 1/50 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)

      Immunohistochemical analysis of Human pancreas tissue labeling TIP30 using ab177961 at 1/50 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)

      Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab177961 at 1/10 dilution to immunoprecipitate TIP30, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-TIP30 antibody [EPR13179] (ab177961)
      Anti-TIP30 antibody [EPR13179] (ab177961)

    References (4)

    Publishing research using ab177961? Please let us know so that we can cite the reference in this datasheet.

    ab177961 has been referenced in 4 publications.

    • Lin X  et al. Long Non-Coding RNA NONHSAT076754 Promotes Invasion and Metastasis in Epithelial Ovarian Cancer. J Cancer 10:1930-1940 (2019). PubMed: 31205552
    • Grund A  et al. TIP30 counteracts cardiac hypertrophy and failure by inhibiting translational elongation. EMBO Mol Med 11:e10018 (2019). PubMed: 31468715
    • Yang Q  et al. Metformin Enhances the Effect of Regorafenib and Inhibits Recurrence and Metastasis of Hepatic Carcinoma After Liver Resection via Regulating Expression of Hypoxia Inducible Factors 2a (HIF-2a) and 30 kDa HIV Tat-Interacting Protein (TIP30). Med Sci Monit 24:2225-2234 (2018). PubMed: 29654226
    • You A  et al. Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models. J Hematol Oncol 9:20 (2016). WB ; Human . PubMed: 26957312

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