Recombinant Anti-TL1A antibody [EPR23330-2] (ab270456)
![Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)](https://www.abcam.com/ps/products/270/ab270456/Images/ab270456-387025-anti-tl1a-antibody-epr233302-western-blot-ht29-mcf7-lysates.jpg "Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23330-2] to TL1A
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TL1A antibody [EPR23330-2]
See all TL1A primary antibodies -
Description
Rabbit monoclonal [EPR23330-2] to TL1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC, HUVEC (treated with/ml TNF-alpha for 24 hours) and HT-29 whole cell lysates. IP: HT-29 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23330-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa).
|
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa). |
|
IP
1/30. |
Target
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Function
Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. -
Tissue specificity
Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Secreted and Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9966 Human
- Omim: 604052 Human
- SwissProt: O95150 Human
- Unigene: 241382 Human
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Alternative names
- MGC129934 antibody
- MGC129935 antibody
- TL1 A antibody
see all
Images
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Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution
Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 25-36 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
TL1A is a glycoprotein, the molecular weight observed is consistent with what has been described in literature (PMID 20675618).
Negative control: MCF7 (PMID: 9434163 ).
This blot was developed using a higher sensitivity ECL substrate.
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![Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] (ab270456)](https://www.abcam.com/ps/products/270/ab270456/Images/ab270456-1-anti-tl1a-antibody-epr233302-immunoprecipitation-ht29.jpg)
Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] (ab270456)TL1A was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate with ab270456 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270456 at 270456 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: ab270456 IP in HT-29 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270456 in mouse ovary tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
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![Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)](https://www.abcam.com/ps/products/270/ab270456/Images/ab270456-387024-anti-tl1a-antibody-epr233302-western-blot-huvec-huvec-treated-withml-tnfalpha-for-24-hours-whole-20-ug-lysates.jpg)
Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg
Lane 2 : HUVEC treated with/ml TNF-alpha for 24 hours, whole cell lysate 20
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 25-36 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 59 seconds.
TL1A is a glycoprotein, the molecular weight observed is consistent with what have been described in literature (PMID 20675618).
The expression of TL1A is induced by TNFa in HUVEC (PMID: 15847792).
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![Anti-TL1A antibody [EPR23330-2] (ab270456)](https://www.abcam.com/ps/products/270/ab270456/Images/ab270456-4-benefits-of-recombinant-antibodies.png)
Anti-TL1A antibody [EPR23330-2] (ab270456)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab270456 has not yet been referenced specifically in any publications.