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RecombinantRabMAb

Recombinant Anti-TLR2 antibody [EPR20302-119] (ab209216)

Reviews (1) Submit a question References (5)
Immunocytochemistry/ Immunofluorescence - Anti-TLR2 antibody [EPR20302-119] (ab209216)
  • Western blot - Anti-TLR2 antibody [EPR20302-119] (ab209216)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)
  • Anti-TLR2 antibody [EPR20302-119] (ab209216)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20302-119] to TLR2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TLR2 antibody [EPR20302-119]
    See all TLR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20302-119] to TLR2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 whole cell lysate. IHC-P: Mouse liver and lung tissues. ICC/IF: RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

Fixation with 4% PFA is not recommended as no staining was obtained.
Notes
WB
1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

Fixation with 4% PFA is not recommended as no staining was obtained.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

  • Tissue specificity

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • Post-translational
    modifications

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-TLR2 antibody [EPR20302-119] (ab209216)
      Immunocytochemistry/ Immunofluorescence - Anti-TLR2 antibody [EPR20302-119] (ab209216)

      Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling TLR2 with ab209216 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in RAW 264.7 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody followed by anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Fixation with 4% PFA is not recommended as no staining was obtained.

       

    • Western blot - Anti-TLR2 antibody [EPR20302-119] (ab209216)
      Western blot - Anti-TLR2 antibody [EPR20302-119] (ab209216)
      Anti-TLR2 antibody [EPR20302-119] (ab209216) at 1/1000 dilution + RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 89 kDa
      Observed band size: 89 kDa


      Exposure time: 26 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling TLR2 with ab209216 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of mouse lung (PMID: 24371556). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab209216 for 10 minutes at 37℃.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (ab209216)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TLR2 with ab209216 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kuffer cells of mouse liver (PMID: 21966468). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab209216 for 10 minutes at 37℃.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-TLR2 antibody [EPR20302-119] (ab209216)
      Anti-TLR2 antibody [EPR20302-119] (ab209216)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (5)

    Publishing research using ab209216? Please let us know so that we can cite the reference in this datasheet.

    ab209216 has been referenced in 5 publications.

    • Zhang M  et al. Upstream stimulatory factor 2 (USF2) induced upregulation of triggering receptor expressed on myeloid cells 1 (TREM1) promotes endometritis by regulating toll-like receptor (TLR) 2/4-nuclear factor-kappaB (NF-κB) signaling pathway. Bioengineered 13:3609-3619 (2022). PubMed: 35100093
    • Mi J  et al. Bacillus subtilis WB800N alleviates diabetic wounds in mice by regulating gut microbiota homeostasis and TLR2. J Appl Microbiol 133:436-447 (2022). PubMed: 35332963
    • Zhu J  et al. Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy. Nat Commun 13:7466 (2022). PubMed: 36463242
    • Xu S  et al. Transcriptome Analysis of Microglia Reveals That the TLR2/IRF7 Signaling Axis Mediates Neuroinflammation After Subarachnoid Hemorrhage. Front Aging Neurosci 13:645649 (2021). PubMed: 34276335
    • Lu X  et al. Celastrol Exerts Cardioprotective Effect in Rheumatoid Arthritis by Inhibiting TLR2/HMGB1 Signaling Pathway-Mediated Autophagy. Int Arch Allergy Immunol 182:1245-1254 (2021). PubMed: 34428765

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-TLR2 antibody [EPR20302-119]

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (oral epithelium)
    Permeabilization
    Yes - triton x-100
    Specification
    oral epithelium
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.553µg/mL · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Amit Leibovich

    Verified customer

    Submitted Jul 12 2019

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