Recombinant Anti-TLR2 antibody [EPR2078Y] (ab68159)
![Western blot - Anti-TLR2 antibody [EPR2078Y] (ab68159)](https://www.abcam.com/ps/products/68/ab68159/Images/ab68159-82370-anti-tlr2-antibody-epr2078y-western-blot.jpg "Western blot - Anti-TLR2 antibody [EPR2078Y] (ab68159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2078Y] to TLR2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TLR2 antibody [EPR2078Y]
See all TLR2 primary antibodies -
Description
Rabbit monoclonal [EPR2078Y] to TLR2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- THP-1 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2078Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
| Notes |
|---|
Application notes
ICC: 1/250 - 1/500.
WB: 1/500 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Is unsuitable for Flow Cyt, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Is unsuitable for Flow Cyt, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
Tissue specificity
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Post-translational
modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7097 Human
- Omim: 603028 Human
- SwissProt: O60603 Human
- Unigene: 519033 Human
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Alternative names
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
see all
Images
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Western blot - Anti-TLR2 antibody [EPR2078Y] (ab68159)Anti-TLR2 antibody [EPR2078Y] (ab68159) at 1/500 dilution + THP-1 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Anti-TLR2 antibody [EPR2078Y] (ab68159)](https://www.abcam.com/ps/products/68/ab68159/Images/ab68159-2-benefits-of-recombinant-antibodies.png)
Anti-TLR2 antibody [EPR2078Y] (ab68159)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab68159 has been referenced in 3 publications.
- Niu X et al. Serum amyloid A 1 induces suppressive neutrophils through the Toll-like receptor 2-mediated signaling pathway to promote progression of breast cancer. Cancer Sci 113:1140-1153 (2022). PubMed: 35102665
- Ding J et al. Role of PIM2 in acute lung injury induced by sepsis. Exp Ther Med 24:543 (2022). PubMed: 35978927
- Al-Sadi R et al. Bifidobacterium bifidum Enhances the Intestinal Epithelial Tight Junction Barrier and Protects against Intestinal Inflammation by Targeting the Toll-like Receptor-2 Pathway in an NF-κB-Independent Manner. Int J Mol Sci 22:N/A (2021). PubMed: 34360835