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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

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  • SDS
  • Certificate of Compliance
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
  • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [28-3] to TMEM119 - Microglial marker
  • Suitable for: IHC-Fr, IHC-P, IHC-FoFr
  • Knockout validated
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-TMEM119 antibody [28-3] - Microglial marker
    See all TMEM119 primary antibodies
  • Description

    Rabbit monoclonal [28-3] to TMEM119 - Microglial marker
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes mouse Tmem119, a transmembrane protein that has been reported to be a highly specific microglia marker that is not expressed by macrophages or other immune or neural cell types (Bennett et al., 2016).
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Recombinant fragment (GST-tag) within Mouse TMEM119 aa 100 to the C-terminus (intracellular). The exact sequence is proprietary.

  • Positive control

    • IHC-Fr: mouse brain, mouse corpus callosum, mouse choroid plexus, spinal cord sections from EAE mice, mouse cerebrum. IHC-P: FFPE mouse brain, mouse cerebrum. IHC-FrFl: Mouse brain IHC-FoFr: Mouse cerebrum.
  • General notes

    This Tmem119 antibody has been knockout validated in IHC, meaning it demonstrated the expected staining in wild type mouse brain sections and no staining was observed in Tmem119 knockout mouse brain sections. This data is shown on this datasheet in the images section. To detect mouse Tmem119 by flow cytometry, we recommend using ab210405. To detect human TMEM119 by IHC, we recommend using ab185333.

    The 28-3 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.

    IHC-Frozen protocol advice:
    For immunohistochemistry on frozen sections, it is recommended that a high concentration of Triton X-100 (0.5%) is used during permeabilization and antibody incubation steps. This may increase the proportion of microglia that stain positive for Tmem119.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    28-3
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • PE Anti-TMEM119 antibody [106-6] (ab225496)
    • Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
    • Anti-TMEM119 antibody [28-3] - BSA and Azide free (ab234501)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (7)
Use a concentration of 0.5 - 1 µg/ml.

We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak.

IHC-P (7)
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Tris-EDTA buffer preferred.

IHC-FoFr (1)
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with citrate buffer pH 6. Incubate the section with primary antibody at 4 ? overnight.

Notes
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.

We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak.

IHC-P
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Tris-EDTA buffer preferred.

IHC-FoFr
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with citrate buffer pH 6. Incubate the section with primary antibody at 4 ? overnight.

Target

  • Cellular localization

    Membrane; Single-pass type I membrane protein
  • Database links

    • Entrez Gene: 231633 Mouse
    • SwissProt: Q8R138 Mouse
    • Alternative names

      • OBIF antibody
      • Osteoblast induction factor antibody
      • PSEC0199 antibody
      • Transmembrane protein 119 antibody
      • UNQ731/PRO1415 antibody
      see all

    Images

    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      IHC image of TMEM119 staining in a section of Mouse cerebrum using ab209064 at 1:50 dilution.The section was fixed with 4% PFA then permeabilized with 0.2% Triton X-100. The secondary antibody was ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1:1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: PBS instead of the primary antibody.

      Positive staining on mouse cerebrum.

    • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      IHC image of TMEM119 staining in a section of frozen normal mouse brain wild type (upper panel) and TMEM119 knockout (lower panel). No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in PBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 0.5 µg/ml. The secondary antibody was ab150087 (shown in red) used at 2 µg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Images were taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      IHC image of TMEM119 and Iba1 co-staining in a section of formalin-fixed paraffin-embedded normal mouse brain. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 1 µg/ml and ab5076 at 5 µg/ml. The secondary antibodies were ab150087 (shown in red) and ab150133 (shown in green) used at 2 µg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Images were taken with a confocal microscope (Leica-Microsystems, TCS SP8).

       

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)This image is courtesy of an anonymous Abreview

      ab209064 staining TMEM119 in Mouse corpus callosum sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody at 1.4µg/ml for 18 hours at 4°C. An Alexa Fluor® 488 -conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

       

      TMEM119 (green), Iba1 (red) and DAPI (blue)

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      IHC image of TMEM119 staining in a section of formalin fixed, paraffin embedded normal mouse brain. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in PBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 0.1 µg/ml. The secondary antibody was ab150087 (shown in red) used at 2 µg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

       

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      IHC image of TMEM119 staining in a section of frozen normal mouse brain. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in PBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 0.5 µg/ml. The secondary antibody was ab150087 (shown in red) used at 2 µg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

       

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      IHC image of TMEM119 staining in a section of formalin fixed, paraffin embedded normal mouse brain, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab209064, 0.5 µg/ml, for 15 mins at room temperature. A goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.

    • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      Normal (WT) mouse brain, stained for TMEM119 (red), Iba1 (green) and DAPI (blue). Samples were baked onto slides for 10 minutes at 60oC, rehydrated with PBS and blocked with blocking buffer (10% serum in PBST). ab209064 at a concentration of 1 µg/mL was incubated with the sample overnight at 4oC. Slides were washed with PBS and a goat anti-rabbit Alexa Fluor 488® was used as the secondary antibody at a concentration of 4 µg/mL.

    • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

      Normal mouse choroid plexus, stained for TMEM119 (red), Iba1 (green) and DAPI (blue). Choroid plexus macrophages are positive for Iba1 and negative for TMEM119. Samples were baked onto slides for 10 minutes at 60oC, rehydrated with PBS and blocked with blocking buffer (10% serum in PBST). ab209064 at a concentration of 1 µg/mL was incubated with the sample overnight at 4oC. Slides were washed with PBS and a goat anti-rabbit Alexa Fluor 488® was used as the secondary antibody at a concentration of 4 µg/mL.

    • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)Image from Sun D et al., EMBO Rep.. 2017;18(10):1801-1816. Fig EV3.; doi: 10.15252/embr.201643668. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Representative FISH analysis of GAS5 (green) co‐stained with ab209064 (red) in spinal cord sections from EAE mice at 30 dpi. Arrows indicate GAS5+TMEM119+ cells. Scale bars = 25 μm.

      Female C57BL/6 mice (6–8 weeks) were deeply anesthetized with 3% chloral hydrate and a laminectomy was performed. After fixing the spine, 1 μl of 1% lysolecithin in a 0.9% sodium chloride solution was injected into the dorsal funiculus at the level of the T11–T12 vertebrae. The day of lysolecithin injection was designated day 0 (0 dpi). The spinal cord around the injection point was isolated and cut into serial cryosections.

      Tissue sections were fixed, permeabilized, and incubated with the primary antibody overnight at 4°C, followed by 2 h of incubation with TRITC‐ or FITC‐conjugated secondary antibodies. Then, the samples were counterstained with Hoechst 33342.

    • Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)Image from Manso Y et al., Glia. 2018;66(1):34-46. Fig 4.; doi: 10.1002/glia.23190 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

      Representative images of sham (d–g) and hypoperfusion (h–k) at 12 weeks post-surgery are shown to illustrate Iba-1 immunostaining in sham (d) and hypoperfused (h); TMEM119 immunostaining in sham (e) and hypoperfused (i) and then Iba-1/TMEM119 co-localisation in sham (f,g) and hypoperfused (j,k) white matter. All Iba-1+ cells in both sham and hypoperfused cohorts were also TMEM119+ indicating that the cells in the corpus callosum were resident microglia. Scale bars; d-f and h-j are 50µm, g and k 10 µm. The number of microglial cells significantly correlated with nodal gap length.

      Free floating cryo-preserved sections cut at 30 μm thickness. Sections were incubated with the primary antibodies (anti-Iba-1 (1/100) and anti-TMEM119 (1/500, ab209064)) overnight at 4°C. Sections were stained at the outset with haematoxylin and eosin to determine the presence and absence of ischemic neuronal perikaryal damage as part of the inclusion/exclusion criteria.

    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (115)

    Publishing research using ab209064? Please let us know so that we can cite the reference in this datasheet.

    ab209064 has been referenced in 115 publications.

    • McNamara NB  et al. Microglia regulate central nervous system myelin growth and integrity. Nature 613:120-129 (2023). PubMed: 36517604
    • Sun ZQ  et al. Lycium barbarum extract promotes M2 polarization and reduces oligomeric amyloid-β-induced inflammatory reactions in microglial cells. Neural Regen Res 17:203-209 (2022). PubMed: 34100457
    • Chen HR  et al. Monocytes promote acute neuroinflammation and become pathological microglia in neonatal hypoxic-ischemic brain injury. Theranostics 12:512-529 (2022). PubMed: 34976198
    • Nucera S  et al. MAFLD progression contributes to altered thalamus metabolism and brain structure. Sci Rep 12:1207 (2022). PubMed: 35075185
    • Guo X  et al. ASK1 signaling regulates phase-specific glial interactions during neuroinflammation. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35101972
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 23 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Syrian hamster Tissue (Brain tissue)
    Permeabilization
    Yes - 1% Triton X-100 in PBS
    Specification
    Brain tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 31 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Blocking Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 27 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 27 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain sections)
    Permeabilization
    No
    Specification
    Brain sections
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Abhijit Nirwane

    Verified customer

    Submitted Apr 16 2020

    Immunohistochemistry free floating abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Mouse Brain Tissue, Microglia)
    Specification
    Mouse Brain Tissue, Microglia
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 12 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, adult)
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Specification
    Testis, adult
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Bryan Niedenberger

    Verified customer

    Submitted Oct 26 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
    Permeabilization
    Yes - Tween-20
    Specification
    brain
    Blocking step
    (agent) for 30 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    10% neutral buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 27 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody [28-3] - Microglial marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted May 16 2019

    1-10 of 23 Abreviews or Q&A

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