Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)
Key features and details
- Rabbit polyclonal to TMEM49/VMP1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM49/VMP1 antibody - C-terminal
See all TMEM49/VMP1 primary antibodies -
Description
Rabbit polyclonal to TMEM49/VMP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Human TMEM49/VMP1 aa 350 to the C-terminus (C terminal). NP_112200.2
Database link: Q96GC9 -
Positive control
- WB: Rat pancreas lysate. ICC/IF: RKO cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/1000. |
|
ICC/IF
1/100. |
Target
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Function
Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis. Plays a role in the initial stages of the autophagic process through its interaction with BECN1 (By similarity). Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions. -
Sequence similarities
Belongs to the VMP1 family. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane. Vacuole membrane. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 508631 Cow
- Entrez Gene: 81671 Human
- Entrez Gene: 75909 Mouse
- Entrez Gene: 100172847 Orangutan
- Entrez Gene: 192129 Rat
- Omim: 611753 Human
- SwissProt: Q0VCK9 Cow
- SwissProt: Q96GC9 Human
see all -
Alternative names
- DKFZP566I133 antibody
- ectopic P-granules autophagy protein 3 homolog antibody
- NF E2 induceble protein 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)
Immunocytochemistry/Immunofluorescence analysis of RKO (Human colon carcinoma cell line) cells labeling TMEM49/VMP1 using ab240887 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain at 1:1000 dilution for 60 min at RT; DAPI (blue) nuclear stain at 1:5000 for 5 min at RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240887 (D) Composite.
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Western blot - Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)16 hours at 4°C at 1/1000 dilution + Rat pancreas cell lysate at 15 µg
Secondary
Goat-Anti-Rabbit IgG: HRP for 60 minutes at RT at 1/200 dilutionBlock: 5% skimmed milk in 1X TBST.
Color development: TMB.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab240887 has not yet been referenced specifically in any publications.