Anti-TMEM97 antibody (ab126490)
Key features and details
- Rabbit polyclonal to TMEM97
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM97 antibody -
Description
Rabbit polyclonal to TMEM97 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: FEDFSKASGFKGQRPETLHERLT, corresponding to amino acids 120-142 of Human TMEM97.
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Positive control
- Human pancreas tissue; Human cell line U-2 OS.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role as a regulator of cellular cholesterol homeostasis. -
Tissue specificity
Widely expressed in normal tissues. Expressed in pancreatic, renal, breast, colon, ovarian surface epithelial (OSE) cells and meningioma cancers. Expressed in ovarian cancer; expression is reduced relative to OSE cells. -
Sequence similarities
Belongs to the TMEM97 family. -
Cellular localization
Nucleus membrane. Rough endoplasmic reticulum. Cell membrane. Lysosome. Membrane. Localized in sterol-depleted cells at cell membrane and in lysosomes. - Information by UniProt
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Database links
- Entrez Gene: 27346 Human
- Omim: 612912 Human
- SwissProt: Q5BJF2 Human
- Unigene: 199695 Human
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Alternative names
- MAC30 antibody
- Protein MAC30 antibody
- Tmem97 antibody
see all
Images
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Western blot - Anti-TMEM97 antibody (ab126490)Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM97 antibody (ab126490)ab126490 at 1/50 dilution staining TMEM97 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126490 has not yet been referenced specifically in any publications.