Recombinant Anti-TMUB1 antibody [EPR14066] (ab180586)
![Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)](https://www.abcam.com/ps/products/180/ab180586/Images/ab180586-465258-anti-tmub1-antibody-epr14066-western-blot-wildtype-hela-tmub1-knockout-hela-hepg2-human-cerebellum.jpg "Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14066] to TMUB1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMUB1 antibody [EPR14066]
See all TMUB1 primary antibodies -
Description
Rabbit monoclonal [EPR14066] to TMUB1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver tissue lysate, Human cerebellum tissue lysate, HeLa cells, HepG2 cells, Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14066 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Detects a band of approximately 24, 27 kDa (predicted molecular weight: 26 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/500.
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| Notes |
|---|
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Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 24, 27 kDa (predicted molecular weight: 26 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/500. |
Target
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Function
May contribute to the regulation of translation during cell-cycle progression. May contribute to the regulation of cell proliferation. -
Sequence similarities
Contains 1 ubiquitin-like domain. -
Cellular localization
Membrane. Cytoplasm. Nucleus. Predominantly nuclear during growth arrest (By similarity). Actively exported from the nucleus in dividing cells. - Information by UniProt
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Database links
- Entrez Gene: 83590 Human
- Entrez Gene: 64295 Mouse
- Entrez Gene: 362301 Rat
- Omim: 614792 Human
- SwissProt: Q9BVT8 Human
- SwissProt: Q9JMG3 Mouse
- SwissProt: Q53AQ4 Rat
- Unigene: 726215 Human
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Alternative names
- C7orf21 antibody
- Chromosome 7 Open Reading Frame 21 antibody
- Dendritic Cell-Derived Ubiquitin-Like Protein antibody
see all
Images
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Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TMUB1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human Cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab180586 was shown to react with TMUB1 in wild-type HeLa cells in Western blot with loss of signal observed in TMUB1 knockout cell line ab278130 (knockout cell lysate ab278185). Wild-type HeLa and TMUB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab180586 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-TMUB1 antibody [EPR14066] (ab180586)](https://www.abcam.com/ps/products/180/ab180586/Images/ab180586-211234-anti-tmub1-antibody-epr14066-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TMUB1 antibody [EPR14066] (ab180586)Intracellular flow cytometric analysis of 2% paraformaldehyde fixedHeLa cells labeling TMUB1 with ab180586 at 1/70 (pink). Control shown in green.
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![Immunocytochemistry/ Immunofluorescence - Anti-TMUB1 antibody [EPR14066] (ab180586)](https://www.abcam.com/ps/products/180/ab180586/Images/ab180586-211232-anti-tmub1-antibody-epr14066-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TMUB1 antibody [EPR14066] (ab180586)Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling TMUB1 with ab180586 at 1/500 (green). Dapi staining shown in blue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMUB1 antibody [EPR14066] (ab180586)](https://www.abcam.com/ps/products/180/ab180586/Images/ab180586-211231-anti-tmub1-antibody-epr14066-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMUB1 antibody [EPR14066] (ab180586)Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TMUB1 with ab180586 at 1/100.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)](https://www.abcam.com/ps/products/180/ab180586/Images/ab180586-414736-recombinant-anti-tmub1-antibody-epr14066-western-blot-hela.jpg)
Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/10000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1- 3: Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab180586 was shown to react with TMUB1 in wild-type HeLa (Human epithelial line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab265852 (knockout cell lysate ab258237) was used. Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) and TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180586 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)](https://www.abcam.com/ps/products/180/ab180586/Images/ab180586-211230-anti-tmub1-antibody-epr14066-western-blot.jpg)
Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/50000 dilution
Lane 1 : Human fetal liver tissue lysate at 20 µg
Lane 2 : Human cerebellum tissue lysate at 20 µg
Lane 3 : HepG2 cell line lysate lysate at 20 µg
Lane 4 : HeLa cell line lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible cleavage fragment) -
![Anti-TMUB1 antibody [EPR14066] (ab180586)](https://www.abcam.com/ps/products/180/ab180586/Images/ab180586-7-benefits-of-recombinant-antibodies.png)
Anti-TMUB1 antibody [EPR14066] (ab180586)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab180586 has been referenced in 2 publications.
- van de Weijer ML et al. Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. Mol Cell 79:768-781.e7 (2020). PubMed: 32738194
- Fu H et al. Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes. Mol Med Rep 17:4337-4344 (2018). PubMed: 29344642