Anti-TNF alpha antibody [2.1_4E10-1H11] (ab9348)
![Sandwich ELISA - Anti-TNF alpha antibody [2.1_4E10-1H11] (ab9348)](https://www.abcam.com/ps/products/9/ab9348/Images/ab9348-400176-AntiTNF-alpha-antibody.png "Sandwich ELISA - Anti-TNF alpha antibody [2.1_4E10-1H11] (ab9348)")
Key features and details
- Mouse monoclonal [2.1_4E10-1H11] to TNF alpha
- Suitable for: Sandwich ELISA, Flow Cyt, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-TNF alpha antibody [2.1_4E10-1H11]
See all TNF alpha primary antibodies -
Description
Mouse monoclonal [2.1_4E10-1H11] to TNF alpha -
Host species
Mouse -
Tested applications
Suitable for: Sandwich ELISA, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human TNF alpha. Recombinant Human TNF-a
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Positive control
- WB: Recombinant human TNF alpha protein (ab9642). Flow Cyt: THP1 cells.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.
Monoclonal antibodies were produced in BALB/c x C57BL/6 F1 mice
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 500µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody was purified from cell culture by Protein G affinity chromatography. -
Clonality
Monoclonal -
Clone number
2.1_4E10-1H11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Affibody® Molecule
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 2 - 4 µg/ml.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-? when used with an appropriate biotinylated antigen affinity purified anti-Human TNF-? as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Sandwich ELISA
Use a concentration of 2 - 4 µg/ml. In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-? when used with an appropriate biotinylated antigen affinity purified anti-Human TNF-? as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7124 Human
- Omim: 191160 Human
- SwissProt: P01375 Human
- Unigene: 241570 Human
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Alternative names
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
Images
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Sandwich ELISA - Anti-TNF alpha antibody [2.1_4E10-1H11] (ab9348)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Human TNF-α when used with suitable detection antibody at a concentration of at least 0.5-1.0 μg/ml.
Datasheets and documents
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Datasheet download
References (4)
ab9348 has been referenced in 4 publications.
- Yang YT et al. Developing Rapid Antimicrobial Susceptibility Testing for Motile/Non-Motile Bacteria Treated with Antibiotics Covering Five Bactericidal Mechanisms on the Basis of Bead-Based Optical Diffusometry. Biosensors (Basel) 10:N/A (2020). PubMed: 33228090
- Jiang T et al. Scolopendra subspinipes mutilans L. Koch Ameliorates Rheumatic Heart Disease by Affecting Relative Percentages of CD4+CD25+FoxP3 Treg and CD4+IL17 T Cells. Evid Based Complement Alternat Med 2019:4674190 (2019). PubMed: 31379962
- Chung CY et al. Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform. Sci Rep 7:46336 (2017). PubMed: 28402317
- Beier EE et al. Alternative microglial activation is associated with cessation of progressive dopamine neuron loss in mice systemically administered lipopolysaccharide. Neurobiol Dis 108:115-127 (2017). PubMed: 28823928
