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Anti-TNF alpha antibody [52B83] (ab1793)

Reviews (18)Q&A (33)References (190)
Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  • Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)

Key features and details

  • Mouse monoclonal [52B83] to TNF alpha
  • Suitable for: ELISA, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr, WB
  • Reacts with: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus
  • Isotype: IgG1

Overview

  • Product name

    Anti-TNF alpha antibody [52B83]
    See all TNF alpha primary antibodies

  • Description

    Mouse monoclonal [52B83] to TNF alpha

  • Host species

    Mouse

  • Specificity

    This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.

  • Tested applications

    Suitable for: ELISA, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr, WBmore details

  • Species reactivity

    Reacts with: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus

  • Immunogen

    Full length native protein (purified) corresponding to Human TNF alpha.

  • Positive control

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-P (5)
Use at an assay dependent concentration.
ICC/IF (2)
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/10.

(methanol fixed cells)

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr (3)
Use at an assay dependent concentration.

Fixed in acetone for 10 minutes
WB (8)
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.

TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.
Notes
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/10.

(methanol fixed cells)

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.

Fixed in acetone for 10 minutes
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.

TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.

Target

  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor (TNF superfamily member 2) antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)
    Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)
    Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)

    ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References (190)

Publishing research using ab1793? Please let us know so that we can cite the reference in this datasheet.

ab1793 has been referenced in 190 publications.

  • Lai Y  et al. Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation. Front Pharmacol 12:616409 (2021). PubMed: 33716743
  • Miao J  et al. Paeonol attenuates inflammation by confining HMGB1 to the nucleus. J Cell Mol Med 25:2885-2899 (2021). PubMed: 33534963
  • Vieira MC  et al. Antinociceptive Effect of Lodenafil Carbonate in Rodent Models of Inflammatory Pain and Spinal Nerve Ligation-Induced Neuropathic Pain. J Pain Res 14:857-866 (2021). PubMed: 33833563
  • Chen J  et al. Evaluation of the Effectiveness of a Chronic Ocular Hypertension Mouse Model Induced by Intracameral Injection of Cross-Linking Hydrogel. Front Med (Lausanne) 8:643402 (2021). PubMed: 33829024
  • Liu Y  et al. Peripherally misfolded proteins exacerbate ischemic stroke-induced neuroinflammation and brain injury. J Neuroinflammation 18:29 (2021). PubMed: 33472658
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF alpha antibody [52B83]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP C42B prostate cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
LNCaP C42B prostate cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Jun 21 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF alpha antibody [52B83]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary astroglial culture)
Specification
Primary astroglial culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 12 2011

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