Anti-TNF alpha antibody [52B83] (ab1793)
![Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.com/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-42.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)")
Key features and details
- Mouse monoclonal [52B83] to TNF alpha
- Suitable for: ELISA, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr, WB
- Reacts with: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus
- Isotype: IgG1
Overview
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Product name
Anti-TNF alpha antibody [52B83]
See all TNF alpha primary antibodies -
Description
Mouse monoclonal [52B83] to TNF alpha -
Host species
Mouse -
Specificity
This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues. -
Tested applications
Suitable for: ELISA, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus -
Immunogen
Full length native protein (purified) corresponding to Human TNF alpha.
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Positive control
- Purchase matching WB positive control:Recombinant Human TNF alpha protein
- Flow Cyt (Intra): RAW 264.7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
52B83 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Affibody® Molecule
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Isotype control
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Recombinant Protein
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Related Products
- Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
- (R,S)-Rolipram, Selective PDE4 inhibitor (ab120029)
- (S)-(+)-Rolipram, PDE4 inhibitor (ab120030)
- (R)-(-)-Rolipram, Selective PDE4 inhibitor (ab120031)
- Human TNF alpha ELISA Kit (ab181421)
- Mouse TNF alpha ELISA Kit (ab208348)
- Human TNF alpha Matched Antibody Pair Kit (ab213467)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| IHC-P | (5) |
Use at an assay dependent concentration.
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| Flow Cyt (Intra) |
1/10.
(methanol fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
Fixed in acetone for 10 minutes |
| WB | (8) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used. |
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt (Intra)
1/10. (methanol fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
Use at an assay dependent concentration. Fixed in acetone for 10 minutes |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used. |
Target
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7124 Human
- Entrez Gene: 21926 Mouse
- Omim: 191160 Human
- SwissProt: Q8HZD9 Chimpanzee
- SwissProt: P79337 Cynomolgus monkey
- SwissProt: P01375 Human
- SwissProt: P06804 Mouse
- SwissProt: P48094 Rhesus monkey
see all -
Alternative names
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
![Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.com/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-12.jpg)
Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.com/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-41.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
![Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.com/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-43.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (238)
ab1793 has been referenced in 238 publications.
- Zheng Y et al. Low-level laser therapy prevents medication-related osteonecrosis of the jaw-like lesions via IL-1RA-mediated primary gingival wound healing. BMC Oral Health 23:14 (2023). PubMed: 36627695
- Zhou T et al. miR-204-containing exosomes ameliorate GVHD-associated dry eye disease. Sci Adv 8:eabj9617 (2022). PubMed: 35020440
- Park HR & Yang EJ Combined Treatment with Herbal Medicine and Drug Ameliorates Inflammation and Metabolic Abnormalities in the Liver of an Amyotrophic Lateral Sclerosis Mouse Model. Antioxidants (Basel) 11:N/A (2022). PubMed: 35052677
- Du J Synergistic effect of Bruton's tyrosine kinase and TNF-α in the regulation of rheumatoid arthritis and underlying mechanisms. Exp Ther Med 23:141 (2022). PubMed: 35069822
- Hu M et al. Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways. Bioengineered 13:3958-3968 (2022). PubMed: 35112986