Anti-TNF alpha antibody [TNFA/1172] (ab220210)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-277566-anti-tnf-alpha-antibody-tnfa-1172-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)")
Key features and details
- Mouse monoclonal [TNFA/1172] to TNF alpha
- Suitable for: IHC-P, ICC
- Reacts with: Rat, Human
- Isotype: IgM
Overview
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Product name
Anti-TNF alpha antibody [TNFA/1172]
See all TNF alpha primary antibodies -
Description
Mouse monoclonal [TNFA/1172] to TNF alpha -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human TNF alpha.
Database link: P01375 -
Positive control
- IHC-P: Human Erdheim Chester disease, rat stomach and rat pancreas tissues; ICC: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
TNFA/1172 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use a concentration of 1 - 2 µg/ml.
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| Notes |
|---|
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7124 Human
- Entrez Gene: 24835 Rat
- Omim: 191160 Human
- SwissProt: P01375 Human
- SwissProt: P16599 Rat
- Unigene: 241570 Human
- Unigene: 2275 Rat
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Alternative names
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat pancreas tissue labeling TNF alpha with ab220210 at 4 μg/mL.
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![Immunocytochemistry - Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-5-anti-tnf-alpha-antibody-tnfa-1172-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry - Anti-TNF alpha antibody [TNFA/1172] (ab220210)Immunofluorescence analysis of PFA-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling TNF alpha with ab220210 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (green). The nuclear counterstain is Redot (red). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-277565-anti-tnf-alpha-antibody-tnfa-1172-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling TNF alpha with ab220210 at 4 μg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-277564-anti-tnf-alpha-antibody-tnfa-1172-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Erdheim Chester disease tissue labeling TNF alpha with ab220210 at 4 μg/mL.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab220210 has been referenced in 11 publications.
- Deng C et al. Targeted apoptosis of macrophages and osteoclasts in arthritic joints is effective against advanced inflammatory arthritis. Nat Commun 12:2174 (2021). PubMed: 33846342
- Makalish TP et al. Anti-Rheumatic Effect of Antisense Oligonucleotide Cytos-11 Targeting TNF-a Expression. Int J Mol Sci 22:N/A (2021). PubMed: 33498456
- Ura K et al. Ultra-purified alginate gel implantation decreases inflammatory cytokine levels, prevents intervertebral disc degeneration, and reduces acute pain after discectomy. Sci Rep 11:638 (2021). PubMed: 33436742
- Qin Z et al. Mild hypothermia in rat with acute myocardial ischaemia-reperfusion injury complicating severe sepsis. J Cell Mol Med N/A:N/A (2021). PubMed: 34057282
- Wu SJ et al. Dexmedetomidine Exerted Anti-arrhythmic Effects in Rat With Ischemic Cardiomyopathy via Upregulation of Connexin 43 and Reduction of Fibrosis and Inflammation. Front Physiol 11:33 (2020). PubMed: 32116751