Anti-TNF Receptor I antibody (ab223352)
Key features and details
- Rabbit polyclonal to TNF Receptor I
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TNF Receptor I antibody
See all TNF Receptor I primary antibodies -
Description
Rabbit polyclonal to TNF Receptor I -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Mouse TNF Receptor I aa 1-100.
Database link: P19438 -
Positive control
- WB: Mouse liver lysate. ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) and HaCaT (human keratinocyte cell line) cells. IHC: Mouse back skin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
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| WB |
1/1000. Predicted molecular weight: 50.5 kDa.
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| IHC-P |
1/100.
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| Notes |
|---|
|
ICC/IF
1/100. |
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WB
1/1000. Predicted molecular weight: 50.5 kDa. |
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IHC-P
1/100. |
Target
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Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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Database links
- Entrez Gene: 7132 Human
- Entrez Gene: 21937 Mouse
- Entrez Gene: 25625 Rat
- Omim: 191190 Human
- SwissProt: P19438 Human
- SwissProt: P25118 Mouse
- SwissProt: P22934 Rat
- Unigene: 279594 Human
see all -
Alternative names
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
Images
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Western blot - Anti-TNF Receptor I antibody (ab223352)Anti-TNF Receptor I antibody (ab223352) at 1/1000 dilution + mouse liver lysate at 15 µg
Secondary
Goat anti-rabbit IgG-HRP at 1/2000 dilution
Predicted band size: 50.5 kDaBlocking: 5% skimmed milk in 1X TBST.
Primary incubation: 2 hours at room temperature
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Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (ab223352)2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (red) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is APC Goat Anti-Rabbit used at 1/200 dilution. The nuclear counter stain is DAPI (blue)(panel A). The merged images are shown in panel C.
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Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (ab223352)2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is FITC Goat Anti-Rabbit used at 1/200 diliution. The nuclear counter stain is DAPI (blue)(panel A) used at 1/40,000 dilution. The merged images are shown in panel C.
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Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (ab223352)Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF. The secondary antibody is FITC Goat Anti-Rabbit at a 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (ab223352)Sections of mouse back skin were stained for TNF Receptor I with ab223352 at a dilution of 1/100 in immunohistochemical analysis.
The secondary antibody was FITC Goat Anti-Rabbit (green) at a 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab223352 has been referenced in 4 publications.
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Fan Y et al. Klotho in Osx+-mesenchymal progenitors exerts pro-osteogenic and anti-inflammatory effects during mandibular alveolar bone formation and repair. Signal Transduct Target Ther 7:155 (2022). PubMed: 35538062
- Moujalled D et al. The necroptotic cell death pathway operates in megakaryocytes, but not in platelet synthesis. Cell Death Dis 12:133 (2021). PubMed: 33510145
- Chen X et al. Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma. Cell Death Discov 7:33 (2021). PubMed: 33597510