Anti-TNF Receptor II antibody [MR2-1] (ab8161)
![Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor II antibody [MR2-1] (ab8161)](https://www.abcam.com/ps/products/8/ab8161/Images/ab8161-13629-anti-tnf-receptor-ii-antibody-mr2-1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor II antibody [MR2-1] (ab8161)")
Key features and details
- Mouse monoclonal [MR2-1] to TNF Receptor II
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TNF Receptor II antibody [MR2-1]
See all TNF Receptor II primary antibodies -
Description
Mouse monoclonal [MR2-1] to TNF Receptor II -
Host species
Mouse -
Specificity
The antibody reacts with the extra-cellular part of the TNF-R II and with the soluble receptor. -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TNF Receptor II (extracellular). Soluble TNF-R75 from culture supernatant of cells transfected with the extracellular part of TNF-R75, purified by affinity chromatography on a TNF-coupled agarose column.
Database link: P20333 -
Positive control
- Recombinant human Soluble TNF Receptor II protein (ab640) can be used as a positive control in WB.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MR2-1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| Flow Cyt | (1) |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7133 Human
- Omim: 191191 Human
- SwissProt: P20333 Human
- Unigene: 256278 Human
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Alternative names
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor II antibody [MR2-1] (ab8161)ICC/IF image of ab8161 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-TNF Receptor II antibody [MR2-1] (ab8161)](https://www.abcam.com/ps/products/8/ab8161/Images/ab8161-13627-anti-tnf-receptor-ii-antibody-mr2-1-flow-cytometry.jpg)
Flow Cytometry - Anti-TNF Receptor II antibody [MR2-1] (ab8161)Overlay histogram showing HL60 cells stained with ab8161 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab8161 has been referenced in 8 publications.
- Ekstrand J et al. Breast Density and Estradiol Are Major Determinants for Soluble TNF-TNF-R Proteins in vivo in Human Breast Tissue. Front Immunol 13:850240 (2022). PubMed: 35432372
- Ji W et al. Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634. J Orthop Translat 36:132-144 (2022). PubMed: 36185580
- Koizumi M et al. P2X3 receptor upregulation in trigeminal ganglion neurons through TNFa production in macrophages contributes to trigeminal neuropathic pain in rats. J Headache Pain 22:31 (2021). PubMed: 33902429
- Jo E et al. Cordyceps militaris induces apoptosis in ovarian cancer cells through TNF-a/TNFR1-mediated inhibition of NF-?B phosphorylation. BMC Complement Med Ther 20:1 (2020). PubMed: 32020859
- Miettinen JA et al. Tumor necrosis factor alpha promotes the expression of immunosuppressive proteins and enhances the cell growth in a human bone marrow-derived stem cell culture. Exp Cell Res 317:791-801 (2011). ICC/IF ; Human . PubMed: 21182837
- Ding B et al. FOXO3a regulates oxygen-responsive expression of tumor necrosis factor receptor 2 in human dermal microvascular endothelial cells. J Biol Chem 284:19331-9 (2009). WB ; Human . PubMed: 19473970
- Till A et al. The Met-196 -> Arg variation of human tumor necrosis factor receptor 2 (TNFR2) affects TNF-alpha-induced apoptosis by impaired NF-kappaB signaling and target gene expression. J Biol Chem 280:5994-6004 (2005). PubMed: 15572357
- Leeuwenberg JF et al. Slow release of soluble TNF receptors by monocytes in vitro. J Immunol 152:4036-43 (1994). PubMed: 8144970
