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RecombinantRabMAb

Recombinant Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

Submit a review Submit a question References (2)
Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
  • Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9692(B)] to TNNC1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TNNC1 antibody [EPR9692(B)]
    See all TNNC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9692(B)] to TNNC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal heart and Human skeletal muscle lysates; Human heart and skeletal muscle tissues

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

  • Involvement in disease

    Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z) [MIM:611879]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNC1 are the cause of cardiomyopathy familial hypertrophic type 13 (CMH13) [MIM:613243]. A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

  • Sequence similarities

    Belongs to the troponin C family.
    Contains 4 EF-hand domains.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cardiac troponin C antibody
      • CMD1Z antibody
      • CMH13 antibody
      • slow skeletal and cardiac muscles antibody
      • Slow twitch skeletal/cardiac muscle troponin C antibody
      • TN-C antibody
      • TNC antibody
      • TNNC antibody
      • Tnnc1 antibody
      • TNNC1 troponin C type 1 (slow) antibody
      • TNNC1_HUMAN antibody
      • Troponin C antibody
      • troponin C type 1 (slow) antibody
      • Troponin C, slow skeletal and cardiac muscles antibody
      • Troponin C1, slow antibody
      see all

    Images

    • Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      All lanes : Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

      Immunohistochemical analysis of paraffin embedded Human heart tissue labelling TNNC1 using ab137130 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

      Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling TNNC1, using ab137130 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

      Immunohistochemical analysis using ab137130 showing positive staining in human Heart tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

      Immunohistochemical analysis using ab137130 showing negative staining in Normal human brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

      Immunohistochemical analysis using ab137130 showing negative staining in Normal human kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

      Immunohistochemical analysis using ab137130 showing negative staining in Normal human colon tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

      Immunohistochemical analysis using ab137130 showing positive staining in human Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
      Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

    References (2)

    Publishing research using ab137130? Please let us know so that we can cite the reference in this datasheet.

    ab137130 has been referenced in 2 publications.

    • Chang H  et al. Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse. J Comp Physiol B 188:863-876 (2018). PubMed: 30039299
    • Chang H  et al. iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus). Proteome Sci 14:16 (2016). WB . PubMed: 27833457

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