Recombinant Anti-TPH2 antibody [EPR25100-29] (ab288067)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25100-29] to TPH2
- Suitable for: WB, IHC-P, IHC-Fr, IP, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-TPH2 antibody [EPR25100-29]
See all TPH2 primary antibodies -
Description
Rabbit monoclonal [EPR25100-29] to TPH2 -
Host species
Rabbit -
Specificity
No suitable positive material of human species is available for in-house IHC-P QC, and it worked well on overexpressed cells pellets of human species.
-
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IP, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human brain, Mouse midbrain, Rat midbrain, HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector with myc-His-tag® whole lysates. IHC-P: Mouse midbrain, Mouse cerebrum, Rat midbrain, Rat cerebrum tissues. IHC-Fr: Mouse midbrain, Rat midbrain tissues. ICC/IF: 293T+OE-1169 cells. Flow Cyt: HEK-293T transfected with a human Tryptophan 5-hydroxylase 2 vector with myc-His tag. IP: HEK-293T transfected with Tryptophan 5-hydroxylase 2 vector with myc-His-tag®, Mouse midbrain cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25100-29 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 56 kDa.
|
|
IHC-P |
1/2000.
|
|
IHC-Fr |
1/500.
|
|
IP |
1/30.
|
|
Flow Cyt |
1/500.
|
|
ICC/IF |
1/500.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 56 kDa. |
IHC-P
1/2000. |
IHC-Fr
1/500. |
IP
1/30. |
Flow Cyt
1/500. |
ICC/IF
1/500. |
Target
-
Tissue specificity
Brain specific. -
Pathway
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
Involvement in disease
Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
-
Database links
- Entrez Gene: 121278 Human
- Entrez Gene: 216343 Mouse
- Entrez Gene: 317675 Rat
- Omim: 607478 Human
- SwissProt: Q8IWU9 Human
- SwissProt: Q8CGV2 Mouse
- SwissProt: Q8CGU9 Rat
- Unigene: 376337 Human
see all -
Alternative names
- MGC138871 antibody
- ADHD7 antibody
- FLJ37295 antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Mouse midbrain tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in mouse midbrain.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat midbrain (fresh) tissue labeling TPH2 with ab288067 at 1/500 (1.26 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on rat midbrain is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
-
All lanes : Anti-TPH2 antibody [EPR25100-29] (ab288067) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containi a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
-
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T+OE-1169 cells labelling TPH2 with ab288067 at 1/500 (1.26 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody ready to use(Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged TPH2 expression vector. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 (0.38ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) is ready to use.
-
TPH2 was immunoprecipitated from 0.35 mg HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate 10 ug with ab288067 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288067 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate 10 ug
Lane 2: ab288067 IP in HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab288067 in HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
-
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Tryptophan 5-hydroxylase 2 expression vector containing a myc-His tag cells labelling TPH2 with ab288067 at 1/500 dilution (0.1ug)( Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse midbrain (fresh) tissue labeling TPH2 with ab288067 at 1/500 (1.26 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on mouse midbrain is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
-
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . No staining in rat kidney.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Rat midbrain tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in rat midbrain.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
All lanes : Anti-TPH2 antibody [EPR25100-29] (ab288067) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: kidney (PMID: 12511643).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
-
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . No staining in mouse kidney.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
TPH2 was immunoprecipitated from 0.35 mg Mouse midbrain tissue lysate 10 ug with ab288067 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288067 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse midbrain tissue lysate 10 ug
Lane 2: ab288067 IP in Mouse midbrain tissue lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab288067 in mouse midbrain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
-
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Cytoplasmic staining on (A) HEK-293T cells transfected with a TPH2 expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in neural fibers of rat cerebrum.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
All lanes : Anti-TPH2 antibody [EPR25100-29] (ab288067) at 1/1000 dilution
Lane 1 : Mouse midbrain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat midbrain tissue lysate
Lane 5 : Rat kidney tissue lysate
Lane 6 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: kidney, spleen (PMID: 12511643).
Exposure time: Lanes 1-3: 70 seconds; Lanes 4-6: 26 seconds
-
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in neural fibers of mouse cerebrum.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab288067 has been referenced in 1 publication.
- Zhao R et al. Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism. Nutrients 14:N/A (2022). PubMed: 35334886