Anti-Tpit antibody [CL6251] (ab243028)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)](https://www.abcam.com/ps/products/243/ab243028/Images/ab243028-331741-anti-tpit-antibody-cl6251-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)")
Key features and details
- Mouse monoclonal [CL6251] to Tpit
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Tpit antibody [CL6251]
See all Tpit primary antibodies -
Description
Mouse monoclonal [CL6251] to Tpit -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Tpit aa 350 to the C-terminus.
Database link: O60806 -
Epitope
Binds to an epitope located within the peptide sequence VLGEPSLTSIAVSTW as determined by overlapping synthetic peptides -
Positive control
- IHC-P: Human pituitary gland tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL6251 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Tpit (TBX19, T box 19) is a highly cell-restricted transcription factor belonging to the T-Box family. T box 19 is required for pro-opeiomelanocortin (POMC) gene expression and differentiation of corticotroph and melanotroph cells. Tpit gene mutations have been shown to be directly correlated to pituitary adrenocorticotropin (ACTH) deficiencies. Tpit functions as a transcriptional activator by recruiting the p160 family of coactivators. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 9095 Human
- Omim: 604614 Human
- SwissProt: O60806 Human
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Alternative names
- DJ747L4.1 antibody
- T Box 19 antibody
- T box factor, pituitary antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)
Formalin-fixed, paraffin-embedded human pituitary gland tissue stained for Tpit with ab243028 at a 1:1000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)](https://www.abcam.com/ps/products/243/ab243028/Images/ab243028-331740-anti-tpit-antibody-cl6251-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)Negative control - Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tpit with ab243028 at a 1:1000 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab243028 has been referenced in 5 publications.
- Dong W et al. CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas. Cells 11:N/A (2022). PubMed: 35954244
- Onizuka H et al. Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT. Acta Histochem Cytochem 54:87-96 (2021). PubMed: 34276102
- Taniguchi-Ponciano K et al. Transcriptome and methylome analysis reveals three cellular origins of pituitary tumors. Sci Rep 10:19373 (2020). PubMed: 33168897
- Taniguchi-Ponciano K et al. Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from POU1F1- and NR5A1-Cell Lineages. Genes (Basel) 11:N/A (2020). PubMed: 33261069
- Torregrosa-Quesada ME et al. How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria? Cancers (Basel) 11:N/A (2019). PubMed: 31835737