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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TPM4 antibody [EPR13317] (ab181156)

Submit a review Submit a question References (1)
Western blot - Anti-TPM4 antibody [EPR13317] (ab181156)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody [EPR13317] (ab181156)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody [EPR13317] (ab181156)
  • Western blot - Anti-TPM4 antibody [EPR13317] (ab181156)
  • Anti-TPM4 antibody [EPR13317] (ab181156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13317] to TPM4
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TPM4 antibody [EPR13317]
    See all TPM4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13317] to TPM4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HeLa and A549 cell lysates. IHC-P: Human cardiac muscle and spleen tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13317

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 28 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 28 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.

  • Tissue specificity

    Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets of hypertensive patients without cardiac hypertrophy (at protein level).

  • Sequence similarities

    Belongs to the tropomyosin family.

  • Domain

    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • epididymis secretory protein Li 108 antibody
    • HEL-S-108 antibody
    • TM30p1 antibody
    • TMP 4 antibody
    • TPM4 antibody
    • TPM4_HUMAN antibody
    • Tropomyosin 4 antibody
    • Tropomyosin alpha 4 chain antibody
    • Tropomyosin alpha-4 chain antibody
    • Tropomyosin-4 antibody
    • wu:fb06e07 antibody
    • zgc:103436 antibody
    • zgc:63909 antibody
    see all

Images

  • Western blot - Anti-TPM4 antibody [EPR13317] (ab181156)
    Western blot - Anti-TPM4 antibody [EPR13317] (ab181156)
    All lanes : Anti-TPM4 antibody [EPR13317] (ab181156) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : TPM4 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 29 kDa why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab181156 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181156 Anti-TPM4 antibody [EPR13317] was shown to specifically react with TPM4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266606 (knockout cell lysate ab257758) was used.  Wild-type and TPM4 knockout samples were subjected to SDS-PAGE.  ab181156 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody [EPR13317] (ab181156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody [EPR13317] (ab181156)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling TPM4 with ab181156 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody [EPR13317] (ab181156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPM4 antibody [EPR13317] (ab181156)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling TPM4 with ab181156 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-TPM4 antibody [EPR13317] (ab181156)
    Western blot - Anti-TPM4 antibody [EPR13317] (ab181156)
    All lanes : Anti-TPM4 antibody [EPR13317] (ab181156) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 29 kDa why is the actual band size different from the predicted?

  • Anti-TPM4 antibody [EPR13317] (ab181156)
    Anti-TPM4 antibody [EPR13317] (ab181156)

References (1)

Publishing research using ab181156? Please let us know so that we can cite the reference in this datasheet.

ab181156 has been referenced in 1 publication.

  • Zhao X  et al. Cytoplasmic Localization Isoform of Cyclin Y Enhanced the Metastatic Ability of Lung Cancer via Regulating Tropomyosin 4. Front Cell Dev Biol 9:684819 (2021). PubMed: 34222253

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