Recombinant Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)](https://www.abcam.com/ps/products/109/ab109503/Images/ab109503-4-anti-transferrin-antibody-epr2932-2-immunohistochemistry-liver-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2932(2)] to Transferrin - Serum Loading Control
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control
See all Transferrin primary antibodies -
Description
Rabbit monoclonal [EPR2932(2)] to Transferrin - Serum Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma lysates; IHC-P: human liver tissue; Flow Cyt (intra): HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2932(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
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Alternative names
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Transferrin with Purified ab109503 at 1:300 dilution (2.19 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)](https://www.abcam.com/ps/products/109/ab109503/Images/ab109503-367555-anti-transferrin-antibody-epr2932-2-westernblot-plasma-human.jpg)
Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503) at 1/10000 dilution (Purified) + Human plasma lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa -
![Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)](https://www.abcam.com/ps/products/109/ab109503/Images/ab109503-5-anti-transferrin-antibody-epr29322-serum-loading-control-flow-cytometry-hepg2.jpg)
Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling Transferrin with ab109503 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)](https://www.abcam.com/ps/products/109/ab109503/Images/ab109503-122400-anti-transferrin-antibody-epr29322-serum-loading-control-western-blot.jpg)
Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)All lanes : Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503) at 1/1000 dilution (unpurified)
Lane 1 : fetal liver lysate
Lane 2 : prostate cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 77 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)](https://www.abcam.com/ps/products/109/ab109503/Images/ab109503-122399-anti-transferrin-antibody-epr29322-serum-loading-control-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)Immunohistochemical analysis of Transferrin expression in formalin-fixed, paraffin-embedded Human liver tissue, using unpurified ab109503 at 1/250 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)](https://www.abcam.com/ps/products/109/ab109503/Images/ab109503-6-benefits-of-recombinant-antibodies.png)
Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab109503 has been referenced in 4 publications.
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Tang Z et al. Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin. Clin Transl Med 11:e390 (2021). PubMed: 33931967
- Lee JH et al. Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis. Clin Proteomics 17:20 (2020). PubMed: 32518534
- Rondon EP et al. Evidence Supporting the Safety of Pegylated Diethylaminoethyl-Chitosan Polymer as a Nanovector for Gene Therapy Applications. Int J Nanomedicine 15:6183-6200 (2020). PubMed: 32922001