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Anti-Transketolase antibody [7H1AA1] (ab112997)

Submit a review Q&A (3)References (6)
Western blot - Anti-Transketolase antibody [7H1AA1] (ab112997)
  • Flow Cytometry - Anti-Transketolase antibody [7H1AA1] (ab112997)
  • Immunocytochemistry/ Immunofluorescence - Anti-Transketolase antibody [7H1AA1] (ab112997)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody [7H1AA1] (ab112997)

Key features and details

  • Mouse monoclonal [7H1AA1] to Transketolase
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 HRP

Overview

  • Product name

    Anti-Transketolase antibody [7H1AA1]
    See all Transketolase primary antibodies

  • Description

    Mouse monoclonal [7H1AA1] to Transketolase

  • Host species

    Mouse

  • Specificity

    Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HeLa and HepG2 whole cell lysate (ab7900); HL60 and HeLa cells. Human normal colon FFPE tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab112997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

Images

  • Western blot - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Western blot - Anti-Transketolase antibody [7H1AA1] (ab112997)
    All lanes : Anti-Transketolase antibody [7H1AA1] (ab112997) at 1 µg/ml

    Lane 1 : HepG2 Cell Lysate
    Lane 2 : HeLa Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 68 kDa

  • Flow Cytometry - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Flow Cytometry - Anti-Transketolase antibody [7H1AA1] (ab112997)
    ab112997 at 1ug/ml staining Transketolase in HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Immunocytochemistry/ Immunofluorescence - Anti-Transketolase antibody [7H1AA1] (ab112997)
    ab112997 at 1ug/ml staining Transketolase in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI (blue). Non specific fluorescence was reduced using a signal enhancer.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody [7H1AA1] (ab112997)

    IHC image of Transketolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112997, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (6)

Publishing research using ab112997? Please let us know so that we can cite the reference in this datasheet.

ab112997 has been referenced in 6 publications.

  • Kraft AM & Petty HR Spatial locations of certain enzymes and transporters within preinvasive ductal epithelial cells predict human breast cancer recurrences. Am J Physiol Cell Physiol 319:C910-C921 (2020). PubMed: 32903032
  • Zheng Y  et al. Hepatitis B virus X protein-induced SH2 domain-containing 5 (SH2D5) expression promotes hepatoma cell growth via an SH2D5-transketolase interaction. J Biol Chem 294:4815-4827 (2019). PubMed: 30659097
  • Tseng CW  et al. Transketolase Regulates the Metabolic Switch to Control Breast Cancer Cell Metastasis via the a-Ketoglutarate Signaling Pathway. Cancer Res 78:2799-2812 (2018). PubMed: 29599405
  • Burrai GP  et al. A first immunohistochemistry study of transketolase and transketolase-like 1 expression in canine hyperplastic and neoplastic mammary lesions. BMC Vet Res 13:38 (2017). PubMed: 28143530
  • Yang H  et al. MicroRNA-497 regulates cisplatin chemosensitivity of cervical cancer by targeting transketolase. Am J Cancer Res 6:2690-2699 (2016). WB, IHC-P . PubMed: 27904781
  • Croner RS  et al. Identification of Predictive Markers for Response to Neoadjuvant Chemoradiation in Rectal Carcinomas by Proteomic Isotope Coded Protein Label (ICPL) Analysis. Int J Mol Sci 17:209 (2016). IHC . PubMed: 26861291

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Product code: 112997
Inquiry: Do you have any information regarding the cross-reactivity in canine formalin fixed tissues (IHC)? ab112997

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Answer

Thank you very much for your interest in Abcam products.

To our knowledge, ab112997 has not been tested in Dog. However the immunogen sequence of ab112997 is quite homologous with Dog species so this ab most likely would cross react. I would encourage you to participate in our Abtrial programme. By participating you can use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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Question

I have been using one of your anti-transketolase antibodies (ab112997) with good success. I have one question regarding the specificity. Is this particular antibody specific for transketolase (TKT) or does it also recognize the other transketolase family members, TKTL-1 and TKTL-2? The product data sheets that accompanied the purchased antibody didn't say and I couldn't find it on the website, either.

Read More
Answer

Thank you for contacting Abcam regarding ab112997.


Our TKT antibody was developed from a shotgun project, thus we do not exactly know the epitope of the antibody. The antibody was validated with Hela cells which express both TKT and TKTL1. IP resulted a single band and confirmed as TKT. No trace of TKTL1 was detected. The sequence similarity between TKT and TKTL1 or TKTL2 is 68% and 63% respectively. I can't ensure you for 100% since we did not test other tissues, but the evidence so far shows this antibody is specific to TKT.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Question

Hi, The storage instructions for this antibody indicates that it should be stored at 4oC and should not be frozen. Could I check with you the reason why this antibody cannot be frozen?

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Answer

Thank you for your inquiry. We advise our customers to store this antibody at 4C. We do have data that storage at 4C will not affect the quality of this antibody. It might well be possible to freeze this antibody without experiencing quality loss and I would suggest to aliquot it to avoid freeze/thaw cycles in this case. But this has not been experimentally tested by us and it is not covered by our Abpromise guarantee. I strongly recommend to store ab112997 as stated on the datasheet to make sure it the Abpromise still applies. More information about our Abpromise guarantee can be found here: https://www.abcam.com/index.html?pageconfig=abpromise I hope this information is helpful. Please do not hesitate to contact me again with any further questions

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