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RecombinantRabMAb

Recombinant Anti-TRBP antibody [EPR13550] (ab180947)

Submit a review Submit a question References (3)
Western blot - Anti-TRBP antibody [EPR13550] (ab180947)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] (ab180947)
  • Immunoprecipitation - Anti-TRBP antibody [EPR13550] (ab180947)
  • Flow Cytometry (Intracellular) - Anti-TRBP antibody [EPR13550] (ab180947)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] (ab180947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBP antibody [EPR13550] (ab180947)
  • Anti-TRBP antibody [EPR13550] (ab180947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13550] to TRBP
  • Suitable for: Flow Cyt (Intra), ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free PE

Overview

  • Product name

    Anti-TRBP antibody [EPR13550]
    See all TRBP primary antibodies

  • Description

    Rabbit monoclonal [EPR13550] to TRBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, HeLa, HepG2 and MCF-7 cell lysates; Human Infiltrating duct carcinoma of breast tissue; HeLa cells; Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50.
IP
1/40.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50.
IP
1/40.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.

  • Sequence similarities

    Contains 3 DRBM (double-stranded RNA-binding) domains.

  • Cellular localization

    Cytoplasm. Cytoplasm > perinuclear region. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • LOQS antibody
    • Prbp antibody
    • RISC loading complex subunit TARBP2 antibody
    • RISC-loading complex subunit tarbp2 antibody
    • TAR (HIV 1) RNA binding protein 2 antibody
    • TAR (HIV) RNA binding protein 2 antibody
    • TAR (HIV) RNA-binding protein TRBP1 antibody
    • TAR RNA Binding Protein 2 antibody
    • TAR RNA-binding protein 2 antibody
    • tarbp2 antibody
    • TARBP2 RISC loading complex RNA binding subunit antibody
    • Trans-activation-responsive RNA-binding protein antibody
    • TRBP antibody
    • TRBP1 antibody
    • TRBP2 antibody
    • TRBP2_HUMAN antibody
    see all

Images

  • Western blot - Anti-TRBP antibody [EPR13550] (ab180947)
    Western blot - Anti-TRBP antibody [EPR13550] (ab180947)
    All lanes : Anti-TRBP antibody [EPR13550] (ab180947) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] (ab180947)
    Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] (ab180947)

    ab180947 staining TRBP antibody in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

  • Immunoprecipitation - Anti-TRBP antibody [EPR13550] (ab180947)
    Immunoprecipitation - Anti-TRBP antibody [EPR13550] (ab180947)

    Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180947 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-TRBP antibody [EPR13550] (ab180947)
    Flow Cytometry (Intracellular) - Anti-TRBP antibody [EPR13550] (ab180947)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat cells labeling TRBP with ab180947 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG Isotype control, followed byGoat anti rabbit IgG (FITC)secondary antibody at 1/150 dilution. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] (ab180947)
    Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] (ab180947)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling TRBP with ab180947 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). DAPI staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBP antibody [EPR13550] (ab180947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBP antibody [EPR13550] (ab180947)

    Immunohistochemical analysis of paraffin-embedded Human Infiltrating duct carcinoma of breast tissue labeling TRBP with ab180947 at 1/250 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-TRBP antibody [EPR13550] (ab180947)
    Anti-TRBP antibody [EPR13550] (ab180947)

References (3)

Publishing research using ab180947? Please let us know so that we can cite the reference in this datasheet.

ab180947 has been referenced in 3 publications.

  • Haikerwal A  et al. Inhibition of Venezuelan Equine Encephalitis Virus Using Small Interfering RNAs. Viruses 14:N/A (2022). PubMed: 35893693
  • Tian T  et al. Long Noncoding RNA MPRL Promotes Mitochondrial Fission and Cisplatin Chemosensitivity via Disruption of Pre-miRNA Processing. Clin Cancer Res 25:3673-3688 (2019). PubMed: 30885939
  • Sherman Horev H  et al. Increase in Processing Factors Is Involved in Skewed MicroRNA Expression in Patients with Ulcerative Colitis Who Develop Small Intestine Inflammation after Pouch Surgery. Inflamm Bowel Dis 24:1045-1054 (2018). PubMed: 29688474

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