Recombinant Anti-TRIM24 antibody [EPR6102] (ab174287)
![Western blot - Anti-TRIM24 antibody [EPR6102] (ab174287)](https://www.abcam.com/ps/products/174/ab174287/Images/ab174287-270758-anti-trim24-antibody-epr6102-western-blot.jpg "Western blot - Anti-TRIM24 antibody [EPR6102] (ab174287)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6102] to TRIM24
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-TRIM24 antibody [EPR6102]
See all TRIM24 primary antibodies -
Description
Rabbit monoclonal [EPR6102] to TRIM24 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide within Human TRIM24 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
Database link: O15164 -
Positive control
- WB: HAP1, HeLa, HEK293 and MCF7 cell lysates. IHC: Human colon tissue and Human endometrial carcinoma tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6102 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene. -
Sequence similarities
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. - Information by UniProt
-
Database links
- Entrez Gene: 8805 Human
- Entrez Gene: 21848 Mouse
- Omim: 603406 Human
- SwissProt: O15164 Human
- SwissProt: Q64127 Mouse
- Unigene: 490287 Human
- Unigene: 41063 Mouse
-
Alternative names
- E3 ubiquitin protein ligase TRIM24 antibody
- E3 ubiquitin-protein ligase Trim24 antibody
- hTIF1 antibody
see all
Images
-
Western blot - Anti-TRIM24 antibody [EPR6102] (ab174287)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TRIM24 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab174287 observed at 130 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab174287 was shown to specifically react with TRIM24 when TRIM24 knockout samples were used. Wild-type andTRIM24 knockout samples were subjected to SDS-PAGE. ab174287 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-TRIM24 antibody [EPR6102] (ab174287)](https://www.abcam.com/ps/products/174/ab174287/Images/ab174287-199802-anti-trim24-antibody-epr6102-western-blot.jpg)
Western blot - Anti-TRIM24 antibody [EPR6102] (ab174287)All lanes : Anti-TRIM24 antibody [EPR6102] (ab174287) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 116 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody [EPR6102] (ab174287)](https://www.abcam.com/ps/products/174/ab174287/Images/ab174287-199803-anti-trim24-antibody-epr6102-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody [EPR6102] (ab174287)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TRIM24 with ab174287 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody [EPR6102] (ab174287)](https://www.abcam.com/ps/products/174/ab174287/Images/ab174287-199804-anti-trim24-antibody-epr6102-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody [EPR6102] (ab174287)Immunohistochemical analysis of paraffin-embedded paraffin-embedded Human endometrial carcinoma tissue labeling TRIM24 using ab174287 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-TRIM24 antibody [EPR6102] (ab174287)](https://www.abcam.com/ps/products/174/ab174287/Images/ab174287-5-benefits-of-recombinant-antibodies.png)
Anti-TRIM24 antibody [EPR6102] (ab174287)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab174287 has been referenced in 2 publications.
- Xu ZM et al. Ginsenoside Rg1 Prevents Doxorubicin-Induced Cardiotoxicity through the Inhibition of Autophagy and Endoplasmic Reticulum Stress in Mice. Int J Mol Sci 19:N/A (2018). PubMed: 30463294
- Wang P et al. TRIM24 siRNA induced cell apoptosis and reduced cell viability in human nasopharyngeal carcinoma cells. Mol Med Rep 18:369-376 (2018). PubMed: 29749443