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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

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Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
  • Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TRK fused gene antibody [EPR8765(2)(B)]
    See all TRK fused gene primary antibodies

  • Description

    Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human TRK fused gene aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal brain tissue lysate, wild-type HAP1, 293T and HeLa cell lysates. IHC-P: Human colon tissue. ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for IP.

Target

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in TFG are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TFG is found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with NTRK1. The TFG sequence is fused to the 3'-end of NTRK1 generating the TRKT3 (TRK-T3) fusion transcript.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ36137 antibody
      • HMSNP antibody
      • OTTHUMP00000214045 antibody
      • OTTHUMP00000214046 antibody
      • OTTHUMP00000214047 antibody
      • OTTHUMP00000214048 antibody
      • Protein TFG antibody
      • SPG57 antibody
      • TF6 antibody
      • TFG antibody
      • TFG_HUMAN antibody
      • TRK fused antibody
      • TRK fused gene antibody
      • TRK fused gene protein antibody
      • TRK-fused gene protein antibody
      • TRKT3 antibody
      • TRKT3 oncogene antibody
      see all

    Images

    • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : TFG knockout HeLa cell lysate
      Lane 3 : HAP-1 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 43 kDa
      Observed band size: 57 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used.  Wild-type and TFG knockout samples were subjected to SDS-PAGE.  ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/10000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : TFG knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 57 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab150428 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TRK fused gene with ab150428 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunofluorescent analysis of HeLa cells labelling TRK fused gene with ab150428 at 1/250 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

      Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab150428 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

      Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150428 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

      Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150428 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

      Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150428 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

      Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150428 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 43 kDa

    • Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
      Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

    References (0)

    Publishing research using ab150428? Please let us know so that we can cite the reference in this datasheet.

    ab150428 has not yet been referenced specifically in any publications.

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