Recombinant Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
![Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)](https://www.abcam.com/ps/products/235/ab235872/Images/ab235872-393660-anti-trk-fused-gene-antibody-epr87652-western-blot-human-knockout-lysate.jpg "Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free
See all TRK fused gene primary antibodies -
Description
Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain tissue lysate, wild-type HAP1, MCF7, HEK293T and HeLa cell lysates. IHC-P: Human colon tissue. ICC/IF: HeLa cells.
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General notes
ab235872 is the carrier-free version of ab150428.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8765(2)(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in TFG are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TFG is found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with NTRK1. The TFG sequence is fused to the 3'-end of NTRK1 generating the TRKT3 (TRK-T3) fusion transcript. - Information by UniProt
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Database links
- Entrez Gene: 10342 Human
- Entrez Gene: 21787 Mouse
- Entrez Gene: 360709 Rat
- Omim: 602498 Human
- SwissProt: Q92734 Human
- Unigene: 518123 Human
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Alternative names
- FLJ36137 antibody
- HMSNP antibody
- OTTHUMP00000214045 antibody
see all
Images
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Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TFG knockout HeLa cell lysate
Lane 3 : HAP-1 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab150428).
Lanes 1-4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.
ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)](https://www.abcam.com/ps/products/235/ab235872/Images/ab235872-369848-anti-trk-fused-gene-antibody-epr87652b-bsa-and-azide-free-western-blot-hap1-wt-tfg-knockout-hela-mcf7.jpg)
Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TFG knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab150428).
Lanes 1 - 4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab150428 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)](https://www.abcam.com/ps/products/235/ab235872/Images/ab235872-3-benefits-of-recombinant-antibodies.png)
Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab235872 has not yet been referenced specifically in any publications.
