Recombinant Anti-TRK fused gene antibody [EPR8766] (ab156866)
![Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-393546-anti-trk-fused-gene-antibody-epr8766-western-blot-human-knockout-lysate.jpg "Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8766] to TRK fused gene
- Suitable for: WB, ICC/IF, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TRK fused gene antibody [EPR8766]
See all TRK fused gene primary antibodies -
Description
Rabbit monoclonal [EPR8766] to TRK fused gene -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain tissue lysate and HeLa, A549, 293T, and wild-type HAP1 cell lysates. IHC-P: Human colon and thyroid carcinoma tissues. ICC/IF: HeLa and MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8766 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
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| ICC/IF |
1/1000.
For unpurified use at 1/100 - 1/250 |
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 43 kDa. |
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ICC/IF
1/1000. For unpurified use at 1/100 - 1/250 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
1/10 - 1/100. |
Target
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Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in TFG are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TFG is found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with NTRK1. The TFG sequence is fused to the 3'-end of NTRK1 generating the TRKT3 (TRK-T3) fusion transcript. - Information by UniProt
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Database links
- Entrez Gene: 10342 Human
- Entrez Gene: 21787 Mouse
- Entrez Gene: 360709 Rat
- Omim: 602498 Human
- SwissProt: Q92734 Human
- Unigene: 518123 Human
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Alternative names
- FLJ36137 antibody
- HMSNP antibody
- OTTHUMP00000214045 antibody
see all
Images
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Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TFG knockout HeLa cell lysate
Lane 3 : HAP-1 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.
ab156866 Anti-TRK fused gene antibody [EPR8766] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. ab156866 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-369816-anti-trk-fused-gene-antibody-epr8766-western-blot-hap1-wt-tfg-knockout-hela-mcf7.jpg)
Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TFG knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab156866 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab156866 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-164038-anti-trk-fused-gene-antibody-epr8766-western-blot.jpg)
Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-261220-anti-trk-fused-gene-antibody-epr8766-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling TRK fused gene with purified ab156866 at 1/1000. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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![Immunoprecipitation - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-164037-anti-trk-fused-gene-antibody-epr8766-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TRK fused gene antibody [EPR8766] (ab156866)Immunoprecipitaion analysis in 293T cell lysate of TRK fused gene immunoprecipitated with ab156866 at 1/10.
Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution + Immunoprecipitation pellets from 293T cell lysate at 10 µg -
![Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-164036-anti-trk-fused-gene-antibody-epr8766-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)Immunofluorescent analysis of HeLa cells labeling TRF fused gene with ab156866 at 1/100. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-164035-anti-trk-fused-gene-antibody-epr8766-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TRF fused gene with ab156966 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-164034-anti-trk-fused-gene-antibody-epr8766-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling TRF fused gene with ab156866 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-164033-anti-trk-fused-gene-antibody-epr8766.jpg)
OI-RD Scanning - Anti-TRK fused gene antibody [EPR8766] (ab156866) -
![Anti-TRK fused gene antibody [EPR8766] (ab156866)](https://www.abcam.com/ps/products/156/ab156866/Images/ab156866-10-benefits-of-recombinant-antibodies.png)
Anti-TRK fused gene antibody [EPR8766] (ab156866)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab156866 has been referenced in 4 publications.
- Inukai R et al. The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B. Int J Mol Sci 22:N/A (2021). PubMed: 33503978
- Carinci M et al. TFG binds LC3C to regulate ULK1 localization and autophagosome formation. EMBO J 40:e103563 (2021). PubMed: 33932238
- Yamamotoya T et al. Trk-fused gene (TFG) regulates pancreatic ß cell mass and insulin secretory activity. Sci Rep 7:13026 (2017). PubMed: 29026155
- Kanadome T et al. The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein. FEBS J 284:56-76 (2017). PubMed: 27813252