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  1. Link

    products/primary-antibodies/trkb-antibody-ab18987.pdf

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Neuroscience Neurology process Growth and Development Neurotrophins
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Anti-TrkB antibody (ab18987)

  • Datasheet
  • SDS
Reviews (9)Q&A (2)References (58)

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Western blot - Anti-TrkB antibody (ab18987)

    Key features and details

    • Rabbit polyclonal to TrkB
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-TrkB antibody [EPR1294] (ab134155)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-TrkB antibody
      See all TrkB primary antibodies
    • Description

      Rabbit polyclonal to TrkB
    • Host species

      Rabbit
    • Specificity

      The immunogen has 75% cross reactivity with TrKA and 68% cross reactivity with TrKC.

    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken
    • Immunogen

      Synthetic peptide corresponding to Human TrkB. Surrounding amino acid 810 of human TrkB.
      Database link: Q16620-1

    • Positive control

      • WB: HL-60 cell lysate.
    • General notes

      This product is manufactured by BioVision, an Abcam company and was previously called 3593 TrkB Antibody. 3593-100 is the same size as the 100 µg size of ab18987.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.03% Proclin 300
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.19% EDTA disodium salt
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Growth and Development
      • Neurotrophins
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Growth factor receptors
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Cellular metabolism
      • Neuroscience
      • Development

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HL-60 whole cell lysate (ab7914)
    • Recombinant Protein

      • Recombinant Human TrkB protein (ab174057)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18987 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (3)
    Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
    Notes
    WB
    Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).

    Target

    • Function

      Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
    • Tissue specificity

      Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Ligand-mediated auto-phosphorylation.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q16620 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4915 Human
      • Entrez Gene: 18212 Mouse
      • Entrez Gene: 25054 Rat
      • Omim: 600456 Human
      • SwissProt: Q16620 Human
      • SwissProt: P15209 Mouse
      • SwissProt: Q63604 Rat
      • Unigene: 494312 Human
      • Unigene: 712776 Human
      • Unigene: 130054 Mouse
      • Unigene: 11246 Rat
      see all
    • Alternative names

      • AI848316 antibody
      • BDNF tropomyosine receptor kinase B antibody
      • BDNF/NT 3 growth factors receptor antibody
      • BDNF/NT-3 growth factors receptor antibody
      • Brain derived neurotrophic factor receptor antibody
      • C030027L06Rik antibody
      • EC 2.7.10.1 antibody
      • GP145 TrkB antibody
      • GP145-TrkB antibody
      • GP145-TrkB/GP95-TrkB antibody
      • GP95 TrkB antibody
      • Neurotrophic receptor tyrosine kinase 2 antibody
      • Neurotrophic tyrosine kinase receptor type 2 antibody
      • Neurotrophin receptor tyrosine kinase type 2 antibody
      • NTRK 2 antibody
      • Ntrk2 antibody
      • NTRK2_HUMAN antibody
      • Obesity, hyperphagia, and developmental delay, included antibody
      • RATTRKB1 antibody
      • Tkrb antibody
      • Trk B antibody
      • Trk-B antibody
      • TRKB antibody
      • TrkB tyrosine kinase antibody
      • TRKB1 antibody
      • Tropomyosin related kinase B antibody
      • tyrosine kinase receptor B antibody
      • Tyrosine receptor kinase B antibody
      see all

    Images

    • Western blot - Anti-TrkB antibody (ab18987)
      Western blot - Anti-TrkB antibody (ab18987)
      Anti-TrkB antibody (ab18987) + HL-60 cell lysate

      Predicted band size: 92 kDa
      Observed band size: >90 kDa why is the actual band size different from the predicted?

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (58)

    Publishing research using ab18987? Please let us know so that we can cite the reference in this datasheet.

    ab18987 has been referenced in 58 publications.

    • Tao J  et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
    • Wei X  et al. Inhibiting BDNF/TrkB.T1 receptor improves resiniferatoxin-induced postherpetic neuralgia through decreasing ASIC3 signaling in dorsal root ganglia. J Neuroinflammation 18:96 (2021). PubMed: 33874962
    • Seo SY  et al. Acupuncture Alleviates Anxiety and 22-kHz Ultrasonic Vocalizations in Rats Subjected to Repeated Alcohol Administration by Modulating the Brain-Derived Neurotrophic Factor/Corticotropin-Releasing Hormone Signaling Pathway. Int J Mol Sci 22:N/A (2021). PubMed: 33919862
    • Marchese MJ  et al. Perfluoroalkyl Substance Exposure and the BDNF Pathway in the Placental Trophoblast. Front Endocrinol (Lausanne) 12:694885 (2021). PubMed: 34394001
    • Gowler PRW  et al. Peripheral brain-derived neurotrophic factor contributes to chronic osteoarthritis joint pain. Pain 161:61-73 (2020). PubMed: 31479064
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-TrkB antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 25 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TrkB antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Feb 13 2017

    Flow Cytometry abreview for Anti-TrkB antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 15 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TrkB antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Whole Brain Sections)
    Specification
    Whole Brain Sections
    Permeabilization
    Yes - 10% Tween-20
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 16 2014

    Western blot abreview for Anti-TrkB antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (PC12 cells (neuronal cells))
    Loading amount
    20 µg
    Specification
    PC12 cells (neuronal cells)
    Treatment
    see below (Additional notes)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gels)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 10 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TrkB antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (PC12 cells (neuronal cells))
    Specification
    PC12 cells (neuronal cells)
    Fixative
    Methanol
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 10 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TrkB antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 19 2011

    Western blot abreview for Anti-TrkB antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mousse Cortical neurons and rat brain)
    Loading amount
    10 µg
    Specification
    Mousse Cortical neurons and rat brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Carles Gil

    Verified customer

    Submitted Jun 03 2011

    Western blot abreview for Anti-TrkB antibody

    Below Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (sebocytes)
    Loading amount
    100 µg
    Specification
    sebocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% TBST
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 21 2009

    Question

    I have never used this primary antibody in an ICC before and I was wondering what would be the "best" concentration to use? I figured I would try a 1:1000, 1:2500, 1:5000, 1:7500, 1:10000 to start off but if you could point me in the right direction it would save me some time!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 11 2012

    Answer

    Thank you for contacting us. We recommend using ab18987 with a concentration of at least 2ug/mL for immunocytochemistry on cultured cells. Given the 0.2mg/mL stock concentration for this antibody, that would be a 1:100 dilution. For the strongest signal, we recommend an overnight incubation with the primary antibody. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Sep 11 2012

    1-10 of 11 Abreviews or Q&A

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