Anti-TrkB antibody (ab18987)
Key features and details
- Rabbit polyclonal to TrkB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TrkB antibody
See all TrkB primary antibodies -
Description
Rabbit polyclonal to TrkB -
Host species
Rabbit -
Specificity
The immunogen has 75% cross reactivity with TrKA and 68% cross reactivity with TrKC.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken
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Immunogen
Synthetic peptide corresponding to Human TrkB. Surrounding amino acid 810 of human TrkB.
Database link: Q16620-1 -
Positive control
- WB: HL-60 cell lysate.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3593 TrkB Antibody. 3593-100 is the same size as the 100 µg size of ab18987.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.19% EDTA disodium salt -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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| Notes |
|---|
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
Target
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4915 Human
- Entrez Gene: 18212 Mouse
- Entrez Gene: 25054 Rat
- Omim: 600456 Human
- SwissProt: Q16620 Human
- SwissProt: P15209 Mouse
- SwissProt: Q63604 Rat
- Unigene: 494312 Human
see all -
Alternative names
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
Images
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Western blot - Anti-TrkB antibody (ab18987)Anti-TrkB antibody (ab18987) + HL-60 cell lysate
Predicted band size: 92 kDa
Observed band size: >90 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (73)
ab18987 has been referenced in 73 publications.
- Mishra S et al. The Alzheimer's gene SORL1 is a regulator of endosomal traffic and recycling in human neurons. Cell Mol Life Sci 79:162 (2022). PubMed: 35226190
- Yoon EJ et al. Improvement of Cognitive Function in Ovariectomized Rats by Human Neural Stem Cells Overexpressing Choline Acetyltransferase via Secretion of NGF and BDNF. Int J Mol Sci 23:N/A (2022). PubMed: 35628371
- Leopold AV et al. A general approach for engineering RTKs optically controlled with far-red light. Nat Methods 19:871-880 (2022). PubMed: 35681062
- Lee G et al. Effects of an Exercise Program Combining Aerobic and Resistance Training on Protein Expressions of Neurotrophic Factors in Obese Rats Injected with Beta-Amyloid. Int J Environ Res Public Health 19:N/A (2022). PubMed: 35805580
- Clarke E et al. A Single Domain Shark Antibody Targeting the Transferrin Receptor 1 Delivers a TrkB Agonist Antibody to the Brain and Provides Full Neuroprotection in a Mouse Model of Parkinson's Disease. Pharmaceutics 14:N/A (2022). PubMed: 35890231