Recombinant Anti-TrkB antibody [EPR1294] (ab134155)
![Western blot - Anti-TrkB antibody [EPR1294] (ab134155)](https://www.abcam.com/ps/products/134/ab134155/Images/ab134155-148434-anti-trkb-antibody-epr1294-western-blot.jpg "Western blot - Anti-TrkB antibody [EPR1294] (ab134155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1294] to TrkB
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TrkB antibody [EPR1294]
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR1294] to TrkB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab195463) -
Positive control
- Fetal brain, Human hippocampus and Human cerebellum lysates; Human brain tissue
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General notes
Mouse, Rat: Internal testing data in Western Blot indicate that this antibody is not suitable for these species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1294 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with Human TrkB peptide (ab195463).
|
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| IP |
1/10 - 1/100.
|
|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with Human TrkB peptide (ab195463). |
|
IP
1/10 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4915 Human
- Omim: 600456 Human
- SwissProt: Q16620 Human
- Unigene: 494312 Human
- Unigene: 712776 Human
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Alternative names
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
Images
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Western blot - Anti-TrkB antibody [EPR1294] (ab134155)All lanes : Anti-TrkB antibody [EPR1294] (ab134155) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Human cerebellum tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)](https://www.abcam.com/ps/products/134/ab134155/Images/ab134155-148433-anti-trkb-antibody-epr1294-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab134155 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)](https://www.abcam.com/ps/products/134/ab134155/Images/ab134155-148432-anti-trkb-antibody-epr1294-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab134155 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)](https://www.abcam.com/ps/products/134/ab134155/Images/ab134155-148431-anti-trkb-antibody-epr1294-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab134155 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)](https://www.abcam.com/ps/products/134/ab134155/Images/ab134155-148430-anti-trkb-antibody-epr1294-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134155 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-TrkB antibody [EPR1294] (ab134155)](https://www.abcam.com/ps/products/134/ab134155/Images/ab134155-6-benefits-of-recombinant-antibodies.png)
Anti-TrkB antibody [EPR1294] (ab134155)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab134155 has been referenced in 2 publications.
- Fleury S et al. Tissue-Specificity of Antibodies Raised Against TrkB and p75NTR Receptors; Implications for Platelets as Models of Neurodegenerative Diseases. Front Immunol 12:606861 (2021). PubMed: 33643311
- Aristizabal Prada ET et al. Tropomyosin receptor kinase: a novel target in screened neuroendocrine tumors. Endocr Relat Cancer 25:547-560 (2018). PubMed: 29563190