Recombinant Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)
![Western blot - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)](https://www.abcam.com/ps/products/248/ab248704/Images/ab248704-410695-anti-trkb-antibody-epr1294-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1294] to TrkB - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TrkB antibody [EPR1294] - BSA and Azide free
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR1294] to TrkB - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab195463) -
General notes
ab248704 is the carrier-free version of ab134155.
Mouse, Rat: Internal testing data in Western Blot indicate that this antibody is not suitable for these species.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1294 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 92 kDa.Can be blocked with Human TrkB peptide (ab195463).
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 92 kDa.Can be blocked with Human TrkB peptide (ab195463). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
Target
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4915 Human
- Omim: 600456 Human
- SwissProt: Q16620 Human
- Unigene: 494312 Human
- Unigene: 712776 Human
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Alternative names
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
Images
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Western blot - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)All lanes : Anti-TrkB antibody [EPR1294] (ab134155) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Human cerebellum tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDaThis data was developed using ab134155, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)](https://www.abcam.com/ps/products/248/ab248704/Images/ab248704-4-anti-trkb-antibody-epr1294-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)This data was developed using ab134155, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab134155 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)](https://www.abcam.com/ps/products/248/ab248704/Images/ab248704-3-anti-trkb-antibody-epr1294-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)This data was developed using ab134155, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab134155 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)](https://www.abcam.com/ps/products/248/ab248704/Images/ab248704-2-anti-trkb-antibody-epr1294-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)This data was developed using ab134155, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab134155 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)](https://www.abcam.com/ps/products/248/ab248704/Images/ab248704-1-anti-trkb-antibody-epr1294-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)This data was developed using ab134155, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134155 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)](https://www.abcam.com/ps/products/248/ab248704/Images/ab248704-6-benefits-of-recombinant-antibodies.png)
Anti-TrkB antibody [EPR1294] - BSA and Azide free (ab248704)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248704 has not yet been referenced specifically in any publications.
