Recombinant Anti-TrkB antibody [EPR17805-146] (ab187041)
![Indirect ELISA - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-9-anti-trkb-antibody-epr17805-146-elisa-mouse-trkb-ntrk2.jpg "Indirect ELISA - Anti-TrkB antibody [EPR17805-146] (ab187041)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17805-146] to TrkB
- Suitable for: Indirect ELISA, ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-TrkB antibody [EPR17805-146]
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR17805-146] to TrkB -
Host species
Rabbit -
Tested applications
Suitable for: Indirect ELISA, ICC/IF, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse TrkB active protein; mouse and rat brain lysates. IHC-P: Mouse and rat cerebral cortex tissues and mouse primary neuron cell. IP: Mouse brain lysate. Indirect ELISA: Mouse TrkB antigen. ICC/IF: mouse primary neuron cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17805-146 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
1/50.
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| WB |
1/5000. Detects a band of approximately 140, 90 kDa (predicted molecular weight: 92 kDa).
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/50.
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| Notes |
|---|
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Indirect ELISA
Use at an assay dependent concentration. |
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ICC/IF
1/50. |
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WB
1/5000. Detects a band of approximately 140, 90 kDa (predicted molecular weight: 92 kDa). |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/50. |
Target
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18212 Mouse
- Entrez Gene: 25054 Rat
- SwissProt: P15209 Mouse
- SwissProt: Q63604 Rat
- Unigene: 130054 Mouse
- Unigene: 11246 Rat
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Alternative names
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
Images
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Indirect ELISA - Anti-TrkB antibody [EPR17805-146] (ab187041)ELISA analysis of Mouse TrkB (Ntrk2) recombinant protein at 1000 ng/ml with ab187041. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-8-anti-trkb-antibody-epr17805-146-immunocytochemistry-mouse-primary-neuron.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] (ab187041)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TrkB with ab187041 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
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![Western blot - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-266962-anti-trkb-antibody-epr17805-146-western-blot.jpg)
Western blot - Anti-TrkB antibody [EPR17805-146] (ab187041)Anti-TrkB antibody [EPR17805-146] (ab187041) at 1/5000 dilution + Mouse TrkB active protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 75-85 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Mouse TrkB active protein contains aa1-429 with a C-terminal poly histidine-tag.
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![Western blot - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-266960-anti-trkb-antibody-epr17805-146-western-blot.jpg)
Western blot - Anti-TrkB antibody [EPR17805-146] (ab187041)All lanes : Anti-TrkB antibody [EPR17805-146] (ab187041) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140,90 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
TrkB is abundantly expressed in the central and peripheral nervous system. The 140 kDa observed MW which is higher than the predicted one is due to the glycosylation modification.
90 kDa corresponds to the truncated form, as observed in the literature (PMID:24860020).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-266959-anti-trkb-antibody-epr17805-146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (ab187041)Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and membrane staining on mouse cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-266958-anti-trkb-antibody-epr17805-146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (ab187041)Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and membrane staining on rat cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-266957-anti-trkb-antibody-epr17805-146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] (ab187041)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: no staining on mouse liver [PMID: 2555172].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-266956-anti-trkb-antibody-epr17805-146-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] (ab187041)TrkB was immunoprecipitated from 1 mg of mouse brain lysate with ab187041 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab187041 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse brain lysate 10µg (Input).
Lane 2: ab187041 IP in mouse brain lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab187041 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-TrkB antibody [EPR17805-146] (ab187041)](https://www.abcam.com/ps/products/187/ab187041/Images/ab187041-7-benefits-of-recombinant-antibodies.png)
Anti-TrkB antibody [EPR17805-146] (ab187041)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (13)
ab187041 has been referenced in 13 publications.
- Liu Y et al. Aquaporin 4 deficiency eliminates the beneficial effects of voluntary exercise in a mouse model of Alzheimer's disease. Neural Regen Res 17:2079-2088 (2022). PubMed: 35142700
- Xue Z et al. Role of BDNF/ProBDNF Imbalance in Postoperative Cognitive Dysfunction by Modulating Synaptic Plasticity in Aged Mice. Front Aging Neurosci 14:780972 (2022). PubMed: 35370607
- Sun X et al. Alleviation of Anxiety/Depressive-Like Behaviors and Improvement of Cognitive Functions by Lactobacillus plantarum WLPL04 in Chronically Stressed Mice. Can J Infect Dis Med Microbiol 2021:6613903 (2021). PubMed: 33603935
- Fu R et al. P2X4 receptor in the dorsal horn contributes to BDNF/TrkB and AMPA receptor activation in the pathogenesis of remifentanil-induced postoperative hyperalgesia in rats. Neurosci Lett 750:135773 (2021). PubMed: 33639220
- Abbate JM et al. Anti-Atherogenic Effect of 10% Supplementation of Anchovy (Engraulis encrasicolus) Waste Protein Hydrolysates in ApoE-Deficient Mice. Nutrients 13:N/A (2021). PubMed: 34206655