Recombinant Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
![Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)](https://www.abcam.com/ps/products/223/ab223155/Images/ab223155-7-anti-trkb-antibody-epr17805-146-elisa-mouse-trkb-ntrk2.jpg "Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17805-146] to TrkB - Low endotoxin, Azide free
- Suitable for: WB, ELISA, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Recombinant fragment
Overview
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Product name
Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR17805-146] to TrkB - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse TrkB active protein; mouse and rat brain lysates. IHC-P: Mouse and rat cerebral cortex tissues and mouse primary neuron cell. IP: Mouse brain lysate. ELISA: Mouse TrkB antigen.
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General notes
ab223155 is the carrier-free version of ab187041.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17805-146 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18212 Mouse
- Entrez Gene: 25054 Rat
- SwissProt: P15209 Mouse
- SwissProt: Q63604 Rat
- Unigene: 130054 Mouse
- Unigene: 11246 Rat
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Alternative names
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
Images
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Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
This data was developed using ab223155, the same antibody clone in a different buffer formulation.
ELISA analysis of Mouse TrkB (Ntrk2) recombinant protein at 1000 ng/ml with ab187041. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)](https://www.abcam.com/ps/products/223/ab223155/Images/ab223155-6-anti-trkb-antibody-epr17805-146-immunocytochemistry-mouse-primary-neuron.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)This data was developed using ab187041, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TrkB with ab187041 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)](https://www.abcam.com/ps/products/223/ab223155/Images/ab223155-321632-anti-trkb-antibody-epr17805-146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and membrane staining on mouse cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)](https://www.abcam.com/ps/products/223/ab223155/Images/ab223155-321631-anti-trkb-antibody-epr17805-146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and membrane staining on rat cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)](https://www.abcam.com/ps/products/223/ab223155/Images/ab223155-321630-anti-trkb-antibody-epr17805-146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: no staining on mouse liver [PMID: 2555172].
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)](https://www.abcam.com/ps/products/223/ab223155/Images/ab223155-321629-anti-trkb-antibody-epr17805-146-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)TrkB was immunoprecipitated from 1 mg of mouse brain lysate with ab187041 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab187041 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse brain lysate 10µg (Input).
Lane 2: ab187041 IP in mouse brain lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab187041 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).
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![Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)](https://www.abcam.com/ps/products/223/ab223155/Images/ab223155-5-benefits-of-recombinant-antibodies.png)
Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab223155 has not yet been referenced specifically in any publications.
