Recombinant Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)
![Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)](https://www.abcam.com/ps/products/229/ab229908/Images/ab229908-396572-AntiTrkB-phospho-Y705-antibody.png "Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22298-67] to TrkB (phospho Y705)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TrkB (phospho Y705) antibody [EPR22298-67]
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR22298-67] to TrkB (phospho Y705) -
Host species
Rabbit -
Specificity
The antibody shows low affinity in the natural tissues of mouse and rat
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Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: ChIP-seq,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with mouse TrkB; HEK-293T transfected with human TrkB; PC-12 cells treated with 100ng/ml NGF, Human brain, hippocampus, cerebellum and spinal cord tissue. Rat brain, hippocampus, P7 thalamus, spinal cord and cerebellum tissue. Mouse brain, hippocampus, P7 thalamus and cerebellum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22298-67 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
|
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| WB |
1/1000. Predicted molecular weight: 92 kDa.
|
| Notes |
|---|
|
Dot blot
1/1000. |
|
WB
1/1000. Predicted molecular weight: 92 kDa. |
Target
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4915 Human
- Entrez Gene: 18212 Mouse
- Entrez Gene: 25054 Rat
- Omim: 600456 Human
- SwissProt: Q16620 Human
- SwissProt: P15209 Mouse
- SwissProt: Q63604 Rat
- Unigene: 494312 Human
see all -
Alternative names
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
Images
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Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/1000 dilution
Lane 1 : Human brain
Lane 2 : Human Hippocampus
Lane 3 : Human cerebellum
Lane 4 : Human spinal cord
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 92 kDa
Exposure time: 5 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Observed MW: 140 kDA
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![Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)](https://www.abcam.com/ps/products/229/ab229908/Images/ab229908-396570-AntiTrkB-phospho-Y705-antibody.png)
Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/1000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse hippocampus
Lane 3 : Mouse P7 thalamus
Lane 4 : Mouse cerebellum
Lane 5 : Rat brain
Lane 6 : Rat hippocampus
Lane 7 : Rat P7 thalamus
Lane 8 : Rat cerebellum
Lane 9 : Mouse P5 cerebral cortex
Lane 10 : Mouse P3 retina
Lane 11 : Mouse P20 retina
Lane 12 : Rat spinal cord
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 92 kDa
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Observed MW: 140 kDA
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![Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)](https://www.abcam.com/ps/products/229/ab229908/Images/ab229908-356463-anti-trkb-phospho-t705-antibody-epr22298-67-western-blot-pc12-rat.jpg)
Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/1000 dilution
Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 2 : PC-12 treated with 100ng/ml NGF (ab9796) for 5 minutes at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The phosphorylation sites are conserved between TrkA and TrkB: Tyr674/675 of TrkA corresponds to Tyr706/707 in TrkB. Therefore, the TrkB (phospho T705) antibody can detect the phosphorylation of TrkA T674/675 in PC-12 cells treated with NGF. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23951412).
Exposure time: 3 minutes
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![Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)](https://www.abcam.com/ps/products/229/ab229908/Images/ab229908-356462-anti-trkb-phospho-t705-antibody-epr22298-67-western-blot-human.jpg)
Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/5000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with human TrkB (WT) expression vector containing a myc-His-tag®, whole cell lysate (Untreated membrane)
Lane 3 : HEK-293T transfected with human TrkB (WT) expression vector containing a myc-His-tag®, whole cell lysate (Phosphatase treated membrane)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24959744). Besides, TrkB auto-phosphorylation was observed when TrkB was over-expressed in HEK293 cells, which has been reported in the literature (PMID: 25632160).
Exposure time: 7 seconds
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![Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)](https://www.abcam.com/ps/products/229/ab229908/Images/ab229908-356461-anti-trkb-phospho-t705-antibody-epr22298-67-western-blot-mouse.jpg)
Western blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/5000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, whole cell lysate
Lane 3 : HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, then treated with 50ng/ml BDNF (ab9794) for 10 minutes, whole cell lysate (Untreated membrane)
Lane 4 : HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, then treated with 50ng/ml BDNF (ab9794) for 10 minutes, whole cell lysate (Phosphatase treated membrane)
Lane 5 : HEK-293T transfected with mouse TrkB (mutant Y705A) expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24959744). Besides, TrkB auto-phosphorylation was observed when TrkB was over-expressed in HEK293 cells, which has been reported in the literature (PMID: 25632160).
Exposure time: 48 seconds
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![Dot Blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)](https://www.abcam.com/ps/products/229/ab229908/Images/ab229908-1-anti-trkb-phospho-t705-antibody-epr22298-67-dot-blot-mouse.jpg)
Dot Blot - Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)Dot blot analysis using ab229908 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: TrkB (phospho T705) peptide (aa697-708)
Lane 2: TrkB (phospho T705) peptide (aa702-712)
Lane 3: TrkB non-phospho peptide (aa697-712)
Blocking/diluting buffer and concentration: 5% NFDM/TBST
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![Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)](https://www.abcam.com/ps/products/229/ab229908/Images/ab229908-11-benefits-of-recombinant-antibodies.png)
Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab229908 has been referenced in 3 publications.
- Wei X et al. Inhibiting BDNF/TrkB.T1 receptor improves resiniferatoxin-induced postherpetic neuralgia through decreasing ASIC3 signaling in dorsal root ganglia. J Neuroinflammation 18:96 (2021). PubMed: 33874962
- Yuan C et al. Bexarotene Improve Depression-Like Behaviour in Mice by Protecting Against Neuro-inflammation and Synaptic Damage. Neurochem Res 45:1500-1509 (2020). PubMed: 32170675
- Xue M et al. Electroacupuncture Modulates Spinal BDNF/Tr?B Signaling Pathway and Ameliorates the Sensitization of Dorsal Horn WDR Neurons in Spared Nerve Injury Rats. Int J Mol Sci 21:N/A (2020). PubMed: 32906633