Recombinant Anti-TrkC antibody [EPR22959-40] (ab240651)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22959-40] to TrkC
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TrkC antibody [EPR22959-40]
See all TrkC primary antibodies -
Description
Rabbit monoclonal [EPR22959-40] to TrkC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt,Flow Cyt (Intra),ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human hypothalamus tissue lysate, Human cerebellum, Human hypothalamus, Mouse brain, Rat brain, Mouse cerebellum, Rat cerebellum, 293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: Human cerebrum, Human astrocytoma, Mouse cerebrum, Rat colon, HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with TrkC expression vector containing a myc-His-tag®. IP: Mouse brain, Rat brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22959-40 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 94 kDa.
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IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
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Notes |
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WB
1/1000. Predicted molecular weight: 94 kDa. |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
Target
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Function
Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties. -
Tissue specificity
Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4916 Human
- Entrez Gene: 18213 Mouse
- Entrez Gene: 29613 Rat
- Omim: 191316 Human
- SwissProt: Q16288 Human
- SwissProt: Q6VNS1 Mouse
- SwissProt: Q03351 Rat
- Unigene: 410969 Human
see all -
Alternative names
- EC 2.7.10.1 antibody
- ETS related protein neurotrophic receptor tyrosine kinase fusion antibody
- ETS related protein neurotrophic receptor tyrosine kinase fusion protein antibody
see all
Images
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All lanes : Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution
Lane 1 : Human hypothalamus tissue lysate at 20 µg
Lane 2 : Human liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 145,100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW are consistent with what has been described in the literature (PMID: 17828769).
Negative control: liver (PMID: 7823156).
Exposure time: 15 seconds
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All lanes : Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human hypothalamus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 145,100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW are consistent with what has been described in the literature (PMID: 17828769).
Exposure time: 8 seconds
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All lanes : Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate at 20 µg
Lane 2 : Mouse liver tissue lysate at 40 µg
Lane 3 : Rat brain tissue lysate at 20 µg
Lane 4 : Rat liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 145,100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW are consistent with what has been described in the literature (PMID: 17828769).
Negative control: liver (PMID: 7823156).
Exposure time: 37 seconds
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All lanes : Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 145,100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW are consistent with what has been described in the literature (PMID: 17828769).
Exposure time: 37 seconds
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All lanes : Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : 293T transfected with human TrkA expression vector containing a myc-His-tag®, whole cell lysate
Lane 4 : 293T transfected with human TrkB expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human TrkA and TrkB.
Exposure time: 5.5 seconds
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Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling TrkC with ab240651 at 1/200 (2.645 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human cerebrum. The section was incubated with ab240651 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling TrkC with ab240651 at 1/200 (2.645 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human astrocytoma. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling TrkC with ab240651 at 1/1000 (0.529 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse cerebrum. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling TrkC with ab240651 at 1/1000 (0.529 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on myenteric plexus of rat colon. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded HEK-293T tissue labelling TrkC with ab240651 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with TrkC expression vector. No staining on (B) HEK-293T transfected with a TrkA expression vector, (C) HEK-293T transfected with TrkB expression vector and (D) HEK-293T transfected with empty vector. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Panel A : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with TrkC expression vector containing a myc-His-tag®
Panel B: HEK-293T transfected with TrkA vector containing a myc-His-tag®.
Panel C: HEK-293T transfected with TrkB vector containing a myc-His-tag®.
Panel D: HEK-293T transfected with empty vector containing a myc-His-tag®Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling TrkC with ab240651 at 1/200 (2.645 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Negative control: No staining in human liver. The section was incubated with ab240651 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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TrkC was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab240651 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240651 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab240651 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240651 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
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TrkC was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab240651 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240651 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10 ug
Lane 2: ab240651 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240651 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab240651 has not yet been referenced specifically in any publications.