Recombinant Anti-TRNP1 antibody [EPR11958] (ab174303)
![Western blot - Anti-TRNP1 antibody [EPR11958] (ab174303)](https://www.abcam.com/ps/products/174/ab174303/Images/ab174303-199495-anti-trnp-antibody-epr11958-western-blot.jpg "Western blot - Anti-TRNP1 antibody [EPR11958] (ab174303)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11958] to TRNP1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TRNP1 antibody [EPR11958]
See all TRNP1 primary antibodies -
Description
Rabbit monoclonal [EPR11958] to TRNP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human TRNP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q6NT89 -
Positive control
- MCF7, Jurkat and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11958 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 23 kDa).
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 23 kDa). |
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IP
1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Relevance
DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex. Regulates neural stem cells proliferation and the production of intermediate neural progenitors and basal radial glial cells affecting the process of cerebral cortex gyrification. May control the proliferation rate of cells by regulating their progression through key cell-cycle transition points. Interacts with TMF1; may regulate TRNP1 proteasomal degradation. -
Cellular localization
Nucleus -
Database links
- Entrez Gene: 388610 Human
- Entrez Gene: 69539 Mouse
- Entrez Gene: 688990 Rat
- SwissProt: Q6NT89 Human
- SwissProt: Q80ZI1 Mouse
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Alternative names
- TRNP1 antibody
- C1orf225 antibody
- TMF-regulated nuclear protein 1 antibody
- TRNP antibody
Images
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Western blot - Anti-TRNP1 antibody [EPR11958] (ab174303)All lanes : Anti-TRNP1 antibody [EPR11958] (ab174303) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands. -
![Immunoprecipitation - Anti-TRNP1 antibody [EPR11958] (ab174303)](https://www.abcam.com/ps/products/174/ab174303/Images/ab174303-199494-anti-trnp-antibody-epr11958-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TRNP1 antibody [EPR11958] (ab174303)Immunoprecipitation. ab174303 at 1/1000 labeling TRNP1 immunoprecipitated from HeLa cell lysate using ab174303 at 1/10.
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![Anti-TRNP1 antibody [EPR11958] (ab174303)](https://www.abcam.com/ps/products/174/ab174303/Images/ab174303-3-benefits-of-recombinant-antibodies.png)
Anti-TRNP1 antibody [EPR11958] (ab174303)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab174303 has been referenced in 1 publication.
- Chen X et al. MiR-17-5p downregulation alleviates apoptosis and fibrosis in high glucose-induced human mesangial cells through inactivation of Wnt/ß-catenin signaling by targeting KIF23. Environ Toxicol 36:1702-1712 (2021). PubMed: 34014023