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RecombinantRabMAb

Recombinant Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)

Reviews (2) Submit a question References (1)
Western blot - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
  • OI-RD Scanning - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
  • Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6353] to Tropomodulin 2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Tropomodulin 2 antibody [EPR6353]
    See all Tropomodulin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6353] to Tropomodulin 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Tropomodulin 2 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Fetal brain and SH-SY5Y cell lysates; Human brain tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Relevance

    Tropomodulin is the actin-capping protein for the slow growing end of filamentous actin. Tropomodulin 2 is a neuronal specific isoform which is the major binding protein to brain tropomyosin in rat.

  • Cellular localization

    Cytoplasm; cytoskeleton

  • Database links

    • Alternative names

      • Tropomodulin 2 antibody
      • MGC39481 antibody
      • N Tmod antibody
      • Neuronal tropomodulin antibody
      • NTMOD antibody
      • TMOD2 antibody
      • tropomodulin 2 (neuronal) antibody
      • tropomodulin 2 neuronal antibody
      see all

    Images

    • Western blot - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
      Western blot - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
      All lanes : Anti-Tropomodulin 2 antibody [EPR6353] (ab124833) at 1/10000 dilution

      Lane 1 : Fetal brain tissue lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)

      ab124833, at 1/250 dilution, staining Tropomodulin 2 in paraffin-embedded Human brain tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
      OI-RD Scanning - Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)
      Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)

    References (1)

    Publishing research using ab124833? Please let us know so that we can cite the reference in this datasheet.

    ab124833 has been referenced in 1 publication.

    • Zhou Q  et al. Key Genes And Pathways Controlled By E2F1 In Human Castration-Resistant Prostate Cancer Cells. Onco Targets Ther 12:8961-8976 (2019). PubMed: 31802906

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tropomodulin 2 antibody [EPR6353]

     Below Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Murine Neuroblastome N1E-115)
    Specification
    Murine Neuroblastome N1E-115
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton, 10min
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 03 2013

    Western blot abreview for Anti-Tropomodulin 2 antibody [EPR6353]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma)
    Loading amount
    20 µg
    Specification
    N1E-115 mouse neuroblastoma
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 25 2013

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