Recombinant Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)](https://www.abcam.com/ps/products/157/ab157215/Images/ab157215-164415-anti-tropomodulin-3-antibody-epr10303b-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10303(B)] to Tropomodulin 3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Tropomodulin 3 antibody [EPR10303(B)]
See all Tropomodulin 3 primary antibodies -
Description
Rabbit monoclonal [EPR10303(B)] to Tropomodulin 3 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 79% identity (92% positives) with Tropomodulin-2. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- C2C12, C6, Raw264.7 and Caco-2 cell lysates. Human heart and Human kidney tissue. Immunoprecipitation pellet from Caco-2 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10303(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the tropomodulin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 29766 Human
- Entrez Gene: 50875 Mouse
- Entrez Gene: 300838 Rat
- Omim: 605112 Human
- SwissProt: Q9NYL9 Human
- SwissProt: Q9JHJ0 Mouse
- Unigene: 4998 Human
- Unigene: 38445 Mouse
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Alternative names
- OTTHUMP00000162615 antibody
- Tmod3 antibody
- TMOD3_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Tropomodulin 3 with ab157215 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)](https://www.abcam.com/ps/products/157/ab157215/Images/ab157215-164414-anti-tropomodulin-3-antibody-epr10303b-western-blot.jpg)
Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)All lanes : Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215) at 1/1000 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)](https://www.abcam.com/ps/products/157/ab157215/Images/ab157215-164413-anti-tropomodulin-3-antibody-epr10303b-western-blot.jpg)
Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215) at 1/1000 dilution + Immunoprecipitation pellet from Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg
Predicted band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)](https://www.abcam.com/ps/products/157/ab157215/Images/ab157215-164412-anti-tropomodulin-3-antibody-epr10303b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Tropomodulin 3 with ab157215 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)](https://www.abcam.com/ps/products/157/ab157215/Images/ab157215-5-benefits-of-recombinant-antibodies.png)
Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab157215 has been referenced in 1 publication.
- Zheng H et al. Tropomodulin 3 modulates EGFR-PI3K-AKT signaling to drive hepatocellular carcinoma metastasis. Mol Carcinog 58:1897-1907 (2019). PubMed: 31313392