Recombinant Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
![Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)](https://www.abcam.com/ps/products/109/ab109505/Images/ab109505-483916-anti-tropomyosin-1-alpha-antibody-epr5158-westernblot-human-mouse-rat.jpg "Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5158] to Tropomyosin 1 (alpha)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Tropomyosin 1 (alpha) antibody [EPR5158]
See all Tropomyosin 1 (alpha) primary antibodies -
Description
Rabbit monoclonal [EPR5158] to Tropomyosin 1 (alpha) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human heart, Mouse heart, and Rat heart tissue lysates, and Recombinant Human TMP1 protein. IHC-P: Human cardiac muscle, Rat cardiac muscle, Mouse cardiac muscle, and Human prostatic hyperplasia tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5158 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
|
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. -
Tissue specificity
Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle. -
Involvement in disease
Defects in TPM1 are the cause of cardiomyopathy familial hypertrophic type 3 (CMH3) [MIM:115196]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TPM1 are the cause of cardiomyopathy dilated type 1Y (CMD1Y) [MIM:611878]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the tropomyosin family. -
Domain
The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7168 Human
- Entrez Gene: 22003 Mouse
- Entrez Gene: 24851 Rat
- Omim: 191010 Human
- SwissProt: P09493 Human
- SwissProt: P58771 Mouse
- SwissProt: P04692 Rat
- Unigene: 133892 Human
see all -
Alternative names
- AA986836 antibody
- AI854628 antibody
- Alpha tropomyosin antibody
see all
Images
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Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)All lanes : Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505) at 1/1000 dilution (Purified)
Lane 1 : Human heart lysate at 15 µg
Lane 2 : Mouse heart lysate at 20 µg
Lane 3 : Rat heart lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)](https://www.abcam.com/ps/products/109/ab109505/Images/ab109505-8-anti-tropomyosin-1-alpha-antibody-epr5158-immunohistochemistry-cardiac-muscle-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)](https://www.abcam.com/ps/products/109/ab109505/Images/ab109505-10-anti-tropomyosin-1-alpha-antibody-epr5158-immunohistochemistry-cardiac-muscle-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)](https://www.abcam.com/ps/products/109/ab109505/Images/ab109505-473364-antitropomyosin-1-alpha-antibody-epr5158.jpg)
Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)All lanes : Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505) at 1/1000 dilution
Lane 1 : MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)
Lane 2 : MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)
Lane 3 : MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)
Lane 4 : GST-tagged Recombinant Human TPM4 protein (Full length)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking and Diluting buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)](https://www.abcam.com/ps/products/109/ab109505/Images/ab109505-9-anti-tropomyosin-1-alpha-antibody-epr5158-immunohistochemistry-cardiac-muscle-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)](https://www.abcam.com/ps/products/109/ab109505/Images/ab109505-7-anti-tropomyosin-1-alpha-antibody-epr5158-immunohistochemistry-prostatic-hyperplasia-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)](https://www.abcam.com/ps/products/109/ab109505/Images/ab109505-5-benefits-of-recombinant-antibodies.png)
Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109505 has been referenced in 2 publications.
- Yoo S et al. INO80 Is Required for the Cell Cycle Control, Survival, and Differentiation of Mouse ESCs by Transcriptional Regulation. Int J Mol Sci 23:N/A (2022). PubMed: 36499727
- Pan H et al. Tropomyosin-1 acts as a potential tumor suppressor in human oral squamous cell carcinoma. PLoS One 12:e0168900 (2017). WB . PubMed: 28182650