Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)
![Western blot - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)](https://www.abcam.com/ps/products/113/ab113692/Images/ab113692-412763-anti-tropomyosin-3-antibody-3d5ah3ab4-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)")
Key features and details
- Mouse monoclonal [3D5AH3AB4] to Tropomyosin 3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Tropomyosin 3 antibody [3D5AH3AB4]
See all Tropomyosin 3 primary antibodies -
Description
Mouse monoclonal [3D5AH3AB4] to Tropomyosin 3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- WB: HEK293T, HDFN, H9C2, H4IIE, HepG2 and MEF cell lysates. Flow Cyt: A431 cells. ICC: HDFn cells. IHC-P: Human heart tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
3D5AH3AB4 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 10 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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| ICC/IF |
Use a concentration of 2 µg/ml.
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| Flow Cyt |
Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
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ICC/IF
Use a concentration of 2 µg/ml. |
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Flow Cyt
Use 0.1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. -
Involvement in disease
Defects in TPM3 are the cause of nemaline myopathy type 1 (NEM1) [MIM:609284]. A form of nemaline myopathy with autosomal dominant or recessive inheritance. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Autosomal dominant nemaline myopathy type 1 is characterized by a moderate phenotype with onset between birth and early second decade of life. Weakness is diffuse and symmetric with slow progression often with need for a wheelchair in adulthood. The autosomal recessive form has onset at birth with moderate-to-severe hypotonia and diffuse weakness. In the most severe cases, death can occur before 2 years. Less severe cases have delayed major motor milestones, and these patients may walk, but often need a wheelchair before 10 years.
Defects in TPM3 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TPM3 is found in thyroid papillary carcinomas. A rearrangement with NTRK1 generates the TRK fusion transcript by fusing the amino end of isoform 2 of TPM3 to the 3'-end of NTRK1. -
Sequence similarities
Belongs to the tropomyosin family. -
Domain
The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7170 Human
- Entrez Gene: 59069 Mouse
- Entrez Gene: 117557 Rat
- Omim: 191030 Human
- SwissProt: P06753 Human
- SwissProt: P21107 Mouse
- SwissProt: Q63610 Rat
- Unigene: 535581 Human
see all -
Alternative names
- Alpha tropomyosin 3 antibody
- Alpha tropomyosin slow skeletal antibody
- CFTD antibody
see all
Images
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Western blot - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)All lanes : Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : TPM3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab113692 observed at 37 kDa. Red - loading control ab52901 observed at kDa.
ab113692 Anti-Tropomyosin 3 antibody [3D5AH3AB4] was shown to specifically react with Tropomyosin 3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266422 (knockout cell lysate ab258245) was used. Wild-type and Tropomyosin 3 knockout samples were subjected to SDS-PAGE. ab113692 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)](https://www.abcam.com/ps/products/113/ab113692/Images/ab113692-238327-anti-tropomyosin-3-antibody-3d5ah3ab4-flow-cytometry.jpg)
Flow Cytometry - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)Overlay histogram showing A431 cells stained with ab113692 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113692, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Western blot - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)](https://www.abcam.com/ps/products/113/ab113692/Images/ab113692-126391-anti-tropomyosin-3-antibody-3d5ah3ab4-western-blot.jpg)
Western blot - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)All lanes : Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692) at 1 µg/ml
Lane 1 : HepG2 (human)
Lane 2 : HDFN (human)
Lane 3 : H9C2 (rat)
Lane 4 : H4IIE (rat)
Lane 5 : MEF (mouse)
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)](https://www.abcam.com/ps/products/113/ab113692/Images/ab113692-126390-anti-tropomyosin-3-antibody-3d5ah3ab4-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)ab113692 stained human fibroblast (HDFn) cells, rat cardiomyocytes (H9C2 cells) and mouse embryo fibroblast (MEF) cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (3D5AH3AB4,5 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG2b (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to cytoskeleton. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)](https://www.abcam.com/ps/products/113/ab113692/Images/ab113692-126389-anti-tropomyosin-3-antibody-3d5ah3ab4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692)IHC image of Tropomyosin 3 staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113692, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab113692 has been referenced in 3 publications.
- Thom CS et al. Tropomyosin 1 genetically constrains in vitro hematopoiesis. BMC Biol 18:52 (2020). PubMed: 32408895
- Yu SB et al. Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis. Mol Med Rep 15:1236-1242 (2017). WB, IHC . PubMed: 28138712
- Singleton DC et al. Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis. Oncogene 0:N/A (2014). PubMed: 25486436