Recombinant Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)
![Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)](https://www.abcam.com/ps/products/207/ab207416/Images/ab207416-276133-anti-trove2-ss-a-antibody-epr20322-western-blot.jpg "Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20322] to TROVE2/SS-A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TROVE2/SS-A antibody [EPR20322]
See all TROVE2/SS-A primary antibodies -
Description
Rabbit monoclonal [EPR20322] to TROVE2/SS-A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, Jurkat, 293T, HeLa, C6, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart, fetal kidney and fetal spleen lysates; Mouse and rat brain, heart, kidney and spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20322 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). |
Target
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Function
RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. May stabilize these RNAs from degradation. -
Sequence similarities
Belongs to the Ro 60 kDa family.
Contains 1 TROVE domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 6738 Human
- Entrez Gene: 20822 Mouse
- Entrez Gene: 304833 Rat
- Omim: 600063 Human
- SwissProt: P10155 Human
- SwissProt: O08848 Mouse
- Unigene: 288178 Human
- Unigene: 40370 Mouse
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Alternative names
- 60 kDa ribonucleoprotein Ro antibody
- 60 kDa Ro protein antibody
- 60 kDa SS A/Ro ribonucleoprotein antibody
see all
Images
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Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)All lanes : Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/10000 dilution
Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : 293T (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)](https://www.abcam.com/ps/products/207/ab207416/Images/ab207416-276131-anti-trove2-ss-a-antibody-epr20322-western-blot.jpg)
Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)All lanes : Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)](https://www.abcam.com/ps/products/207/ab207416/Images/ab207416-276130-anti-trove2-ss-a-antibody-epr20322-western-blot.jpg)
Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)All lanes : Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)](https://www.abcam.com/ps/products/207/ab207416/Images/ab207416-276129-anti-trove2-ss-a-antibody-epr20322-western-blot.jpg)
Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)All lanes : Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate
Lane 5 : Mouse brain lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Mouse spleen lysate
Lane 8 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-7: 30 seconds; Lane 8: 3 minutes.
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![Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)](https://www.abcam.com/ps/products/207/ab207416/Images/ab207416-5-benefits-of-recombinant-antibodies.png)
Anti-TROVE2/SS-A antibody [EPR20322] (ab207416)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab207416 has not yet been referenced specifically in any publications.