Name: Anti-TRP1 antibody [EPR21960]
Brand: RabMAb
SKU: ab235447
Rating: 5 (2 reviews)

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Western blot - Anti-TRP1 antibody [EPR21960] (ab235447)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21960] to TRP1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Product name

Anti-TRP1 antibody [EPR21960]
See all TRP1 primary antibodies
Description

Rabbit monoclonal [EPR21960] to TRP1
Host species

Rabbit
Specificity

We recommend WB for human and mouse; we predict it will also work in WB with rat lysates but were unable to test this.
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human skin and melanoma tissues; Mouse and rat skin tissues. WB: MeWo and B16-F0 whole cell lysates. ICC/IF: MeWo and B16-F0 cells. Flow Cyt (intra): MeWo and B16-F0 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21960
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human TRP1 protein (His tag) (ab219285)
Related Products
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab235447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa). We recommend WB for human and mouse; we predict it will also work in WB with rat lysates but were unable to test this.
IHC-P	(1)	1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(1)	1/1000.
Flow Cyt (Intra)		1/60.

Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa). We recommend WB for human and mouse; we predict it will also work in WB with rat lysates but were unable to test this.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/1000.
Flow Cyt (Intra) 1/60.

Function

Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
Tissue specificity

Pigment cells.
Pathway

Pigment biosynthesis; melanin biosynthesis.
Involvement in disease

Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
Sequence similarities

Belongs to the tyrosinase family.
Cellular localization

Melanosome membrane.
Target information above from: UniProt accession P17643 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7306 Human
- Entrez Gene: 22178 Mouse
- Entrez Gene: 298182 Rat
- Omim: 115501 Human
- SwissProt: P17643 Human
- SwissProt: P07147 Mouse
- Unigene: 270279 Human
- Unigene: 30438 Mouse
Alternative names
- 5 antibody
- 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
- 6-dihydroxyindole-2-carboxylic acid oxidase antibody
- b-PROTEIN antibody
- CAS2 antibody
- Catalase B antibody
- CATB antibody
- DHICA oxidase antibody
- Glycoprotein 75 antibody
- GP75 antibody
- Melanoma antigen gp75 antibody
- OCA3 antibody
- TRP antibody
- TRP-1 antibody
- TRP1 antibody
- Tyrosinase related protein 1 antibody
- Tyrosinase-related protein 1 antibody
- TYRP antibody
- TYRP1 antibody
- TYRP1_HUMAN antibody
- TYRRP antibody
see all

Western blot - Anti-TRP1 antibody [EPR21960] (ab235447)

All lanes : Anti-TRP1 antibody [EPR21960] (ab235447) at 1/1000 dilution

Lane 1 : MeWo (human malignant melanoma fibroblast cell ine) whole cell lysate
Lane 2 : B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: Lane 1: 10 seconds; Lane 2: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The observed mass is greater than the predicted due to glycosylation.

The molecular mass observed is consistent with what has been described in the literature (PMID: 21324755; PMID: 18042623;18650849).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of melanocytes in human skin (PMID:21562572). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunofluorescent analysis of 100% methanol fixed B16-F0 (mouse melanoma epithelial cell-like cell line) cells labeling TRP1 with ab235447 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in the B16-F0 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR21960] (ab235447)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized B16-F0 (mouse melanoma epithelial cell-like cell line) cells labeling TRP1 with ab235447 at 1/60 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of melanocytes in rat skin (PMID: 21562572). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of melanocytes in mouse skin (PMID: 21562572). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in tumor cells of human melanoma (PMID: 22045183; 26068396). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR21960] (ab235447)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MeWo (human malignant melanoma fibroblast cell line) cells labeling TRP1 with ab235447 at 1/60 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

TRP1 is predominantly expressed in mature melanosomes (PMID: 27073483, 11266471).
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunofluorescent analysis of 100% methanol fixed MeWo (human malignant melanoma fibroblast cell line) cells labeling TRP1 with ab235447 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in MeWo cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-TRP1 antibody [EPR21960] (ab235447)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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Publishing research using ab235447? Please let us know so that we can cite the reference in this datasheet.

ab235447 has been referenced in 12 publications.

Wu X et al. Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo. Pigment Cell Melanoma Res 35:328-341 (2022). PubMed: 35218147
Yang CY et al. UVB-Induced Secretion of IL-1β Promotes Melanogenesis by Upregulating TYR/TRP-1 Expression In Vitro. Biomed Res Int 2022:8230646 (2022). PubMed: 35572734
Tian H et al. Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration. iScience 25:105050 (2022). PubMed: 36185374
Hong C et al. Epimedium brevicornum Maxim. Extract exhibits pigmentation by melanin biosynthesis and melanosome biogenesis/transfer. Front Pharmacol 13:963160 (2022). PubMed: 36249817
Zhang Y et al. Establishment and validation of evaluation models for post-inflammatory pigmentation abnormalities. Front Immunol 13:991594 (2022). PubMed: 36389813

View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (melonocytes)

Permeabilization

Yes - PBS 0.5% Tween

Specification

melonocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 22°C

Fixative

Methanol

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 26 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (reconstruted human epidermis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Permeabilization

Yes - PBS Tween

Specification

reconstruted human epidermis

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Fixative

Methanol

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 26 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Pathway

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (12)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [EPR21960]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TRP1 antibody [EPR21960]