Recombinant Anti-TRPV1 antibody [N221/17] (ab305178)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)](https://www.abcam.com/ps/products/305/ab305178/Images/ab305178-1-anti-trpv1-antibody-n22117-immunohistochemistry-rat-dorsal-root-gang.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [N221/17] to TRPV1
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-TRPV1 antibody [N221/17]
See all TRPV1 primary antibodies -
Description
Mouse monoclonal [N221/17] to TRPV1 -
Host species
Mouse -
Specificity
Not suitable for mouse IHC-Fr samples.
-
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human, mouse and rat dorsal root ganglia tissue. IHC-Fr : Rat DRG (fresh).
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
N221/17 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IHC-Fr |
1/50.
|
| Notes |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/50. |
Target
-
Function
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis. -
Tissue specificity
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats. -
Domain
The association domain (AD) is necessary for self-association. -
Post-translational
modificationsPhosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane. - Information by UniProt
-
Database links
- Entrez Gene: 7442 Human
- Entrez Gene: 193034 Mouse
- Entrez Gene: 83810 Rat
- Omim: 602076 Human
- SwissProt: Q8NER1 Human
- SwissProt: Q704Y3 Mouse
- SwissProt: O35433 Rat
- Unigene: 579217 Human
see all -
Alternative names
- Capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglia tissue labeling TRPV1 with AB305178 at 1/100 dilution (9.85 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on rat dorsal root ganglia.
The section was incubated with ab305178 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)](https://www.abcam.com/ps/products/305/ab305178/Images/ab305178-2-anti-trpv1-antibody-n22117-immunohistochemistry-mouse-dorsal-root-ga.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglia tissue labeling TRPV1 with AB305178 at 1/100 dilution (9.85 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on mouse dorsal root ganglia.
The section was incubated with ab305178 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)](https://www.abcam.com/ps/products/305/ab305178/Images/ab305178-3-anti-trpv1-antibody-n22117-immunohistochemistry-human-dorsal-root-ga.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)Immunohistochemical analysis of paraffin-embedded human dorsal root ganglia tissue labeling TRPV1 with AB305178 at 1/100 dilution (9.85 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on human dorsal root ganglia.
The section was incubated with ab305178 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)](https://www.abcam.com/ps/products/305/ab305178/Images/ab305178-4-anti-trpv1-antibody-n22117-immunohistochemistry-rat-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [N221/17] (ab305178)Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling TRPV1 with AB305178 at 1/100 dilution (9.85 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining on rat cardiac muscle.
The section was incubated with ab305178 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [N221/17] (ab305178)](https://www.abcam.com/ps/products/305/ab305178/Images/ab305178-5-anti-trpv1-antibody-n22117-immunohistochemistry-frozen-rat-drg-fresh.jpg)
Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [N221/17] (ab305178)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat DRG (fresh) tissue labeling TRPV1 with AB305178 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on rat DRG.
The section was incubated with ab305178 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
-
![Anti-TRPV1 antibody [N221/17] (ab305178)](https://www.abcam.com/ps/products/305/ab305178/Images/ab305178-496246-BenefitsofRecombinantantibodies.jpg)
Anti-TRPV1 antibody [N221/17] (ab305178)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab305178 has not yet been referenced specifically in any publications.