Anti-Trypsin antibody [2C4] (ab17263)
![Western blot - Anti-Trypsin antibody [2C4] (ab17263)](https://www.abcam.com/ps/products/17/ab17263/Images/ab17263-26008-anti-trypsin-antibody-2c4-western-blot.jpg "Western blot - Anti-Trypsin antibody [2C4] (ab17263)")
Key features and details
- Mouse monoclonal [2C4] to Trypsin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Trypsin antibody [2C4]
See all Trypsin primary antibodies -
Description
Mouse monoclonal [2C4] to Trypsin -
Host species
Mouse -
Specificity
This antibody is specific for human trypsin, trypsinogen and trypsin in complex with alpha 1 antitrypsin. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein purified from human pancreatic tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2C4 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 24 kDa.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa. |
Target
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Function
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
Involvement in disease
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5644 Human
- Entrez Gene: 5645 Human
- Entrez Gene: 5646 Human
- Omim: 276000 Human
- Omim: 601564 Human
- Omim: 613578 Human
- SwissProt: P07477 Human
- SwissProt: P07478 Human
see all -
Alternative names
- Alpha-trypsin chain 2 antibody
- Anionic trypsinogen antibody
- Beta trypsin antibody
see all
Images
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Western blot - Anti-Trypsin antibody [2C4] (ab17263)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab17263 has been referenced in 1 publication.
- Yamamoto K et al. Picoliter enzyme reactor on a nanofluidic device exceeding the bulk reaction rate. Analyst 145:5801-5807 (2020). PubMed: 32692324