Recombinant Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
![Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)](https://www.abcam.com/ps/products/210/ab210797/Images/ab210797-259342-anti-tubulin-antibody-epr13796-loading-control-western-blot.jpg "Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13796] to Tubulin - Loading Control
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
Related conjugates and formulations
Overview
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Product name
Anti-Tubulin antibody [EPR13796] - Loading Control
See all Tubulin primary antibodies -
Description
Rabbit monoclonal [EPR13796] to Tubulin - Loading Control -
Host species
Rabbit -
Specificity
ab210797 recognizes Tubulin alpha-1 and alpha-3 chains. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saccharomyces cerevisiae whole cell lysates; full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13796 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsUndergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Alternative names
- Tubulin beta 2b antibody
- Alpha tubulin antibody
- Alpha-tubulin ubiquitous antibody
see all
Images
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Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution + Saccharomyces cerevisiae whole cell lysates at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)](https://www.abcam.com/ps/products/210/ab210797/Images/ab210797-259340-anti-tubulin-antibody-epr13796-loading-control-western-blot.jpg)
Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution + Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain contains aa1-445 with a His-Tag® was made in house.
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![Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)](https://www.abcam.com/ps/products/210/ab210797/Images/ab210797-3-benefits-of-recombinant-antibodies.png)
Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (25)
ab210797 has been referenced in 25 publications.
- Fang Y et al. Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer. Front Oncol 11:565676 (2021). PubMed: 33777731
- Zhang J et al. TGIF1 Knockdown Inhibits the Proliferation and Invasion of Gastric Cancer via AKT Signaling Pathway. Cancer Manag Res 13:2603-2612 (2021). PubMed: 33776478
- Dai Y & Gao X Inhibition of cancer cell-derived exosomal microRNA-183 suppresses cell growth and metastasis in prostate cancer by upregulating TPM1. Cancer Cell Int 21:145 (2021). PubMed: 33653339
- Hong B et al. Oncolytic HSV Therapy Modulates Vesicular Trafficking Inducing Cisplatin Sensitivity and Antitumor Immunity. Clin Cancer Res 27:542-553 (2021). PubMed: 33087329
- Huang JQ et al. SRPK1/AKT axis promotes oxaliplatin-induced anti-apoptosis via NF-?B activation in colon cancer. J Transl Med 19:280 (2021). PubMed: 34193174