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  1. Link

    products/primary-antibodies/tubulin-antibody-epr13796-loading-control-ab210797.pdf

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Isotype/Loading Controls Loading Controls Tubulin
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RecombinantRabMAb

Recombinant Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (25)

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Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
  • Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
  • Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13796] to Tubulin - Loading Control
  • Suitable for: WB
  • Reacts with: Saccharomyces cerevisiae

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Overview

  • Product name

    Anti-Tubulin antibody [EPR13796] - Loading Control
    See all Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR13796] to Tubulin - Loading Control
  • Host species

    Rabbit
  • Specificity

    ab210797 recognizes Tubulin alpha-1 and alpha-3 chains.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Saccharomyces cerevisiae whole cell lysates; full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13796
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Microbiology
    • Organism
    • Eukaryotic Microorganism
    • Yeast
    • Saccharomyces spp

Associated products

  • Alternative Versions

    • Anti-Tubulin antibody [EPR13796] - BSA and Azide free (ab251523)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Notes
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68363 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

Images

  • Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
    Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
    Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution + Saccharomyces cerevisiae whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
    Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
    Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution + Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain contains aa1-445 with a His-Tag® was made in house.

  • Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)
    Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (25)

Publishing research using ab210797? Please let us know so that we can cite the reference in this datasheet.

ab210797 has been referenced in 25 publications.

  • Fang Y  et al. Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer. Front Oncol 11:565676 (2021). PubMed: 33777731
  • Zhang J  et al. TGIF1 Knockdown Inhibits the Proliferation and Invasion of Gastric Cancer via AKT Signaling Pathway. Cancer Manag Res 13:2603-2612 (2021). PubMed: 33776478
  • Dai Y & Gao X Inhibition of cancer cell-derived exosomal microRNA-183 suppresses cell growth and metastasis in prostate cancer by upregulating TPM1. Cancer Cell Int 21:145 (2021). PubMed: 33653339
  • Hong B  et al. Oncolytic HSV Therapy Modulates Vesicular Trafficking Inducing Cisplatin Sensitivity and Antitumor Immunity. Clin Cancer Res 27:542-553 (2021). PubMed: 33087329
  • Huang JQ  et al. SRPK1/AKT axis promotes oxaliplatin-induced anti-apoptosis via NF-?B activation in colon cancer. J Transl Med 19:280 (2021). PubMed: 34193174
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-Tubulin antibody [EPR13796] - Loading Control

Inconclusive
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (B-cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
1e+006 cells
Specification
B-cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 26 2018

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