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    products/primary-antibodies/tweakrfn14-antibody-epr3179-ab109365.pdf

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RecombinantRabMAb

Recombinant Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (16)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
  • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
  • Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3179] to TWEAKR/FN14
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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View more associated products

Overview

  • Product name

    Anti-TWEAKR/FN14 antibody [EPR3179]
    See all TWEAKR/FN14 primary antibodies
  • Description

    Rabbit monoclonal [EPR3179] to TWEAKR/FN14
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3179
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Cancer
    • Growth factors
    • TNF

Associated products

  • Alternative Versions

    • Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
    • Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP
1/40.

For unpurified use at 1/10.

IHC-P (3)
1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP
1/40.

For unpurified use at 1/10.

IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • Tissue specificity

    Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • Sequence similarities

    Contains 1 TNFR-Cys repeat.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9NP84 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51330 Human
    • Entrez Gene: 27279 Mouse
    • Entrez Gene: 302965 Rat
    • Omim: 605914 Human
    • SwissProt: Q9NP84 Human
    • SwissProt: Q9CR75 Mouse
    • Unigene: 355899 Human
    • Unigene: 28518 Mouse
    • Alternative names

      • CD 266 antibody
      • CD266 antibody
      • CD266 antigen antibody
      • FGF inducible 14 antibody
      • FGF-inducible 14 antibody
      • Fibroblast growth factor inducible immediate early response protein 14 antibody
      • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
      • FN 14 antibody
      • FN14 antibody
      • TNFRSF 12A antibody
      • TNFRSF12A antibody
      • TNR12_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 12A antibody
      • TWEAK R antibody
      • Tweak receptor antibody
      • Tweak-receptor antibody
      • TweakR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

      ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

      Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

      Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysate
      Lane 2 : HT-1080 (human fibrosarcoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14-17 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer 5% NFDM /TBST
    • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
      Lane 2 : L6 (rat skeletal muscle) whole cell lysate
      Lane 3 : C2C12 (mouse muscle) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14-17 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer 5% NFDM /TBST
    • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

      ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

      Lane 1 (input): HUVEC whole cell lysate 10ug

      Lane 2 (+): ab109365 + HUVEC whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 14 kDa

    • Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
      Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab109365? Please let us know so that we can cite the reference in this datasheet.

    ab109365 has been referenced in 16 publications.

    • Xu J  et al. Ubiquitously specific protease 4 inhibitor-Vialinin A attenuates inflammation and fibrosis in S100-induced hepatitis mice through Rheb/mTOR signalling. J Cell Mol Med 25:1140-1150 (2021). PubMed: 33295107
    • Yao Y  et al. Prognostic value of novel immune-related genomic biomarkers identified in head and neck squamous cell carcinoma. J Immunother Cancer 8:N/A (2020). PubMed: 32719094
    • Wang X  et al. Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis. Mediators Inflamm 2019:2945083 (2019). PubMed: 31885495
    • Xiao G  et al. Ginkgo Flavonol Glycosides or Ginkgolides Tend to Differentially Protect Myocardial or Cerebral Ischemia-Reperfusion Injury via Regulation of TWEAK-Fn14 Signaling in Heart and Brain. Front Pharmacol 10:735 (2019). PubMed: 31333457
    • Méndez-Barbero N  et al. A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis. EBioMedicine 46:274-289 (2019). PubMed: 31395500
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-TWEAKR/FN14 antibody [EPR3179]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS-Triton 0.25%
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 02 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TWEAKR/FN14 antibody [EPR3179]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
    Permeabilization
    No
    Specification
    skin tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 16 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TWEAKR/FN14 antibody [EPR3179]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered normal formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 16 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TWEAKR/FN14 antibody [EPR3179]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6
    Permeabilization
    No
    Specification
    bone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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