Recombinant Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-122128-anti-tweakr-antibody-epr3179-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3179] to TWEAKR/FN14
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TWEAKR/FN14 antibody [EPR3179]
See all TWEAKR/FN14 primary antibodies -
Description
Rabbit monoclonal [EPR3179] to TWEAKR/FN14 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3179 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
|
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| IP |
1/40.
For unpurified use at 1/10. |
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| IHC-P | (3) |
1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
|
IP
1/40. For unpurified use at 1/10. |
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IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. -
Tissue specificity
Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51330 Human
- Entrez Gene: 27279 Mouse
- Entrez Gene: 302965 Rat
- Omim: 605914 Human
- SwissProt: Q9NP84 Human
- SwissProt: Q9CR75 Mouse
- Unigene: 355899 Human
- Unigene: 28518 Mouse
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Alternative names
- CD 266 antibody
- CD266 antibody
- CD266 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-266767-anti-tweakr-antibody-epr3179-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-266766-anti-tweakr-antibody-epr3179-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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![Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-266756-anti-tweakr-antibody-epr3179-western-blot.jpg)
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysate
Lane 2 : HT-1080 (human fibrosarcoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14-17 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer 5% NFDM /TBST -
![Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-266757-anti-tweakr-antibody-epr3179-western-blot.jpg)
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 2 : L6 (rat skeletal muscle) whole cell lysate
Lane 3 : C2C12 (mouse muscle) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14-17 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer 5% NFDM /TBST -
![Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-266799-anti-tweakr-antibody-epr3179-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).
Lane 1 (input): HUVEC whole cell lysate 10ug
Lane 2 (+): ab109365 + HUVEC whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-122129-anti-tweakr-antibody-epr3179-western-blot.jpg)
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa -
![Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.com/ps/products/109/ab109365/Images/ab109365-8-benefits-of-recombinant-antibodies.png)
Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab109365 has been referenced in 16 publications.
- Xu J et al. Ubiquitously specific protease 4 inhibitor-Vialinin A attenuates inflammation and fibrosis in S100-induced hepatitis mice through Rheb/mTOR signalling. J Cell Mol Med 25:1140-1150 (2021). PubMed: 33295107
- Yao Y et al. Prognostic value of novel immune-related genomic biomarkers identified in head and neck squamous cell carcinoma. J Immunother Cancer 8:N/A (2020). PubMed: 32719094
- Wang X et al. Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis. Mediators Inflamm 2019:2945083 (2019). PubMed: 31885495
- Xiao G et al. Ginkgo Flavonol Glycosides or Ginkgolides Tend to Differentially Protect Myocardial or Cerebral Ischemia-Reperfusion Injury via Regulation of TWEAK-Fn14 Signaling in Heart and Brain. Front Pharmacol 10:735 (2019). PubMed: 31333457
- Méndez-Barbero N et al. A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis. EBioMedicine 46:274-289 (2019). PubMed: 31395500