Anti-Twist antibody (ab50581)
Key features and details
- Rabbit polyclonal to Twist
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Twist antibody
See all Twist primary antibodies -
Description
Rabbit polyclonal to Twist -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Chimpanzee -
Immunogen
Synthetic peptide:
PADDSLSNSEEEPDRQ
conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 12-27 of Human Twist -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (3) |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. -
Tissue specificity
Subset of mesodermal cells. -
Involvement in disease
Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7291 Human
- Entrez Gene: 22160 Mouse
- Omim: 601622 Human
- SwissProt: Q8MI03 Chimpanzee
- SwissProt: Q15672 Human
- SwissProt: P26687 Mouse
- Unigene: 66744 Human
- Unigene: 3280 Mouse
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Alternative names
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
see all
Images
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Human melanoma Mel15 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 2 µg/ml ab50581. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
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Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (ab50581)Image courtesy of an anonymous Abreview.ab50581 staining Twist in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (152)
ab50581 has been referenced in 152 publications.
- Xie T et al. LHX2 facilitates the progression of nasopharyngeal carcinoma via activation of the FGF1/FGFR axis. Br J Cancer 127:1239-1253 (2022). PubMed: 35864158
- Wang YN et al. Shenkang injection improves chronic kidney disease by inhibiting multiple renin-angiotensin system genes by blocking the Wnt/β-catenin signalling pathway. Front Pharmacol 13:964370 (2022). PubMed: 36059935
- Song WP et al. Prognostic and clinicopathological value of Twist expression in esophageal cancer: A meta-analysis. World J Gastrointest Oncol 14:1874-1886 (2022). PubMed: 36187399
- Lou J et al. LncRNA MEG8 plays an oncogenic role in hepatocellular carcinoma progression through miR-367-3p/14-3-3ζ/TGFβR1 axis. Neoplasma 68:273-282 (2021). PubMed: 33147050
- Neufert C et al. Inducible mouse models of colon cancer for the analysis of sporadic and inflammation-driven tumor progression and lymph node metastasis. Nat Protoc 16:61-85 (2021). PubMed: 33318692