Recombinant Anti-Twist Antibody [RP23040206] (ab313483)
![Western blot - Anti-Twist Antibody [RP23040206] (ab313483)](https://www.abcam.com/ps/products/313/ab313483/Images/ab313483-512862-Anti-Twist-Antibody-western-blot-hela-jurkat-molt4-raji-ramos-k562-human.jpg "Western blot - Anti-Twist Antibody [RP23040206] (ab313483)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RP23040206] to Twist
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-Twist Antibody [RP23040206]
See all Twist primary antibodies -
Description
Rabbit recombinant multiclonal [RP23040206] to Twist -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Twist aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q15672 -
Positive control
- ICC/IF: Ntera2 cells WB: HeLa cell lysate, Jurkat cell lysate, MOLT4 cell lysate, Raji cell lysate, Ramos cell lysate, K562 cell lysate
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
RP23040206 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab313483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. |
Target
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Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. -
Tissue specificity
Subset of mesodermal cells. -
Involvement in disease
Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7291 Human
- Omim: 601622 Human
- SwissProt: Q15672 Human
- Unigene: 66744 Human
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Alternative names
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
see all
Images
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Western blot - Anti-Twist Antibody [RP23040206] (ab313483)All lanes : Anti-Twist Antibody [RP23040206] (ab313483) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : Ramos cell lysate
Lane 6 : K562 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Twist Antibody [RP23040206] (ab313483)](https://www.abcam.com/ps/products/313/ab313483/Images/ab313483-1-Anti-Twist-Antibody-icc-if-Ntera2-cells-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Twist Antibody [RP23040206] (ab313483)Immunofluorescent analysis of Ntera2 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous TWIST1 with ab313483 at 2 µg/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of TWIST1 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TWIST1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab313483 has not yet been referenced specifically in any publications.