Anti-Twist antibody [Twist2C1a] (ab50887)
![Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)](https://www.abcam.com/ps/products/50/ab50887/Images/ab50887-396167-Mouse-monoclonal-Twist2C1a-to-Twist-western-blot-A2058-NIH3T3-F2409-human-mouse-rat.jpg "Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)")
Key features and details
- Mouse monoclonal [Twist2C1a] to Twist
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Twist antibody [Twist2C1a]
See all Twist primary antibodies -
Description
Mouse monoclonal [Twist2C1a] to Twist -
Host species
Mouse -
Specificity
Good controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react. Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC. -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Twist (C terminal).
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Positive control
- Purchase matching WB positive control:Recombinant Human Twist2 protein
- WB: Recombinant Human Twist protein (ab132349) can be used as a positive control. A2058, NIH/3T3 and F2408 whole cell lysate. ICC: HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
Twist2C1a -
Isotype
IgG1 -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (9) |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa.
Abcam recommends using BSA blocking. |
| ICC | (1) |
Use a concentration of 2 - 100 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa. Abcam recommends using BSA blocking. |
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ICC
Use a concentration of 2 - 100 µg/ml. |
Target
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Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. -
Tissue specificity
Subset of mesodermal cells. -
Involvement in disease
Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7291 Human
- Entrez Gene: 22160 Mouse
- Entrez Gene: 85489 Rat
- Omim: 601622 Human
- SwissProt: Q15672 Human
- SwissProt: P26687 Mouse
- Unigene: 66744 Human
- Unigene: 3280 Mouse
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Alternative names
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
see all
Images
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Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)All lanes : Anti-Twist antibody [Twist2C1a] (ab50887) at 2 µg/ml
Lane 1 : A2058 (Human melanoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : F2408 (Rat normal fibroblast cell line) whole cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 21 kDa -
![Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)](https://www.abcam.com/ps/products/50/ab50887/Images/ab50887-396166-Mouse-monoclonal-Twist2C1a-to-Twist-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)4% paraformaldehyde-fixed, 0.1% Triton-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Twist using ab50887 at 100 µg/ml in immunocytochemistry.
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![Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)](https://www.abcam.com/ps/products/50/ab50887/Images/ab50887-62625-anti-twist-antibody-twist2c1a-chip-grade-western-blot.jpg)
Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)This image is courtesy of an Abreview submitted by Pawel MazurAll lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C
Lanes 2, 3, 5, 6, 8 & 9: Whole cell lysate of mouse pancreatic tumor
Lanes 1, 4 & 7: Whole cell lysate of Mouse metastatic pancreatic carcinoma
Lysates at 50 µg per lane
Secondary Antibody: An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique
Blocking Step: 10% Milk for 1 hour at room temperature -
![Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)](https://www.abcam.com/ps/products/50/ab50887/Images/ab50887-262238-anti-twist-antibody-twist2c1a-chip-grade-immunofluorescence.jpg)
Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)Loebel DA et al., PLoS One, 9, e98945, 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody
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![Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)](https://www.abcam.com/ps/products/50/ab50887/Images/ab50887-62626-anti-twist-antibody-twist2c1a-chip-grade-immunofluorescence.jpg)
Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat anti-mouse IgG at 1/200 dilution.
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![Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)](https://www.abcam.com/ps/products/50/ab50887/Images/ab50887-62621-anti-twist-antibody-twist2c1a-chip-grade-western-blot.jpg)
Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)Lanes 2-5 : Anti-Twist antibody [Twist2C1a] (ab50887) at 1/50 dilution
Lane 1 : Marker lane
Lane 2 : Hela whole cell lysate, 25µg
Lane 3 : A2058 whole cell lysate, 25µg
Lane 4 : NIH3T3 whole cell lysate, 25µg
Lane 5 : F2408 whole cell lysate, 25µg
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
SDS-PAGE with 10-20% gradient gel. -
![Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)](https://www.abcam.com/ps/products/50/ab50887/Images/ab50887-62623-anti-twist-antibody-twist2c1a-chip-grade-immunofluorescence.jpg)
Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)This image is courtesy of an anonymous Abreviewab50887 staining Twist in a rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (176)
ab50887 has been referenced in 176 publications.
- Li J et al. METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell Death Dis 13:723 (2022). WB ; Human . PubMed: 35985997
- Seok JK et al. Oxidized Phospholipids in Tumor Microenvironment Stimulate Tumor Metastasis via Regulation of Autophagy. Cells 10:N/A (2021). PubMed: 33806593
- Chu Z et al. COE Inhibits Vasculogenic Mimicry by Targeting EphA2 in Hepatocellular Carcinoma, a Research Based on Proteomics Analysis. Front Pharmacol 12:619732 (2021). PubMed: 33867982
- Harn HI et al. Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice. Nat Commun 12:2595 (2021). PubMed: 33972536
- Yan G et al. LncRNA ILF3-AS1 promotes cell migration, invasion and EMT process in hepatocellular carcinoma via the miR-628-5p/MEIS2 axis to activate the Notch pathway. Dig Liver Dis N/A:N/A (2021). PubMed: 34053876