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    products/primary-antibodies/twist-antibody-twist2c1a-ab50887.pdf

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Anti-Twist antibody [Twist2C1a] (ab50887)

  • Datasheet
  • SDS
Reviews (26)Q&A (17)References (176)

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Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)
  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)
  • Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
  • Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)

Key features and details

  • Mouse monoclonal [Twist2C1a] to Twist
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Twist antibody [Twist2C1a]
    See all Twist primary antibodies
  • Description

    Mouse monoclonal [Twist2C1a] to Twist
  • Host species

    Mouse
  • Specificity

    Good controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react. Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC.
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Twist (C terminal).

  • Positive control

    • Purchase matching WB positive control:Recombinant Human Twist2 protein
    • WB: Recombinant Human Twist protein (ab132349) can be used as a positive control. A2058, NIH/3T3 and F2408 whole cell lysate. ICC: HeLa cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    Twist2C1a
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Muscle

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Recombinant Human Twist protein (ab132349)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Twist protein (ab132349)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab50887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (9)
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA blocking.

ICC (1)
Use a concentration of 2 - 100 µg/ml.
Notes
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA blocking.

ICC
Use a concentration of 2 - 100 µg/ml.

Target

  • Function

    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • Tissue specificity

    Subset of mesodermal cells.
  • Involvement in disease

    Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7291 Human
    • Entrez Gene: 22160 Mouse
    • Entrez Gene: 85489 Rat
    • Omim: 601622 Human
    • SwissProt: Q15672 Human
    • SwissProt: P26687 Mouse
    • Unigene: 66744 Human
    • Unigene: 3280 Mouse
    • Alternative names

      • ACS3 antibody
      • B-HLH DNA binding protein antibody
      • bHLHa38 antibody
      • BPES2 antibody
      • BPES3 antibody
      • Class A basic helix-loop-helix protein 38 antibody
      • CRS antibody
      • CRS1 antibody
      • CSO antibody
      • H-twist antibody
      • OTTHUMP00000116043 antibody
      • SCS antibody
      • Twist basic helix loop helix transcription factor 1 antibody
      • Twist family bHLH transcription factor 1 antibody
      • Twist homolog 1 (Drosophila) antibody
      • Twist homolog 1 antibody
      • TWIST homolog of drosophila antibody
      • Twist related protein 1 antibody
      • Twist-related protein 1 antibody
      • TWIST1 antibody
      • TWST1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)
      Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)
      All lanes : Anti-Twist antibody [Twist2C1a] (ab50887) at 2 µg/ml

      Lane 1 : A2058 (Human melanoma cell line) whole cell lysate
      Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
      Lane 3 : F2408 (Rat normal fibroblast cell line) whole cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 21 kDa

    • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)
      Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)

      4% paraformaldehyde-fixed, 0.1% Triton-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Twist using ab50887 at 100 µg/ml in immunocytochemistry.

    • Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
      Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)This image is courtesy of an Abreview submitted by Pawel Mazur
      All lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C

      Lanes 2, 3, 5, 6, 8 & 9: Whole cell lysate of mouse pancreatic tumor
      Lanes 1, 4 & 7: Whole cell lysate of Mouse metastatic pancreatic carcinoma

      Lysates at 50 µg per lane

      Secondary Antibody: An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique

      Blocking Step: 10% Milk for 1 hour at room temperature

      See Abreview

    • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
      Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)Loebel DA et al., PLoS One, 9, e98945, 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody

    • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
      Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)

      ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat anti-mouse IgG at 1/200 dilution.

    • Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
      Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
      Lanes 2-5 : Anti-Twist antibody [Twist2C1a] (ab50887) at 1/50 dilution

      Lane 1 : Marker lane
      Lane 2 : Hela whole cell lysate, 25µg
      Lane 3 : A2058 whole cell lysate, 25µg
      Lane 4 : NIH3T3 whole cell lysate, 25µg
      Lane 5 : F2408 whole cell lysate, 25µg

      Predicted band size: 21 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      SDS-PAGE with 10-20% gradient gel.
    • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
      Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)This image is courtesy of an anonymous Abreview

      ab50887 staining Twist in a rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.

      See Abreview

    Protocols

    • ChIP protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (176)

    Publishing research using ab50887? Please let us know so that we can cite the reference in this datasheet.

    ab50887 has been referenced in 176 publications.

    • Li J  et al. METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell Death Dis 13:723 (2022). WB ; Human . PubMed: 35985997
    • Seok JK  et al. Oxidized Phospholipids in Tumor Microenvironment Stimulate Tumor Metastasis via Regulation of Autophagy. Cells 10:N/A (2021). PubMed: 33806593
    • Chu Z  et al. COE Inhibits Vasculogenic Mimicry by Targeting EphA2 in Hepatocellular Carcinoma, a Research Based on Proteomics Analysis. Front Pharmacol 12:619732 (2021). PubMed: 33867982
    • Harn HI  et al. Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice. Nat Commun 12:2595 (2021). PubMed: 33972536
    • Yan G  et al. LncRNA ILF3-AS1 promotes cell migration, invasion and EMT process in hepatocellular carcinoma via the miR-628-5p/MEIS2 axis to activate the Notch pathway. Dig Liver Dis N/A:N/A (2021). PubMed: 34053876
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 43 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Term placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 5.7
    Permeabilization
    No
    Specification
    Term placenta
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Arthur Colson

    Verified customer

    Submitted Sep 13 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (orthotopic tumor tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Low pH
    Permeabilization
    Yes - TritonX100 0.025% 10min RT
    Specification
    orthotopic tumor tumor
    Blocking step
    MOM kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Clara Lubeseder-Martellato

    Verified customer

    Submitted Jun 13 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Aug 07 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Human Cell (HEK 293T)
    Specification
    HEK 293T
    Permeabilization
    Yes - Triton 1%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 18 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Sample
    Dog Tissue sections (MDCK pellets in paraffin)
    Specification
    MDCK pellets in paraffin
    Permeabilization
    Yes - Tween-20
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 04 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Twist [Twist2C1a] antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    Human Cell (skin)
    Specification
    skin
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eunhye Kang

    Verified customer

    Submitted Jun 12 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Human Cell (oral squamous cell carcinoma)
    Specification
    oral squamous cell carcinoma
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 10 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris based pH 9.5
    Sample
    Human Tissue sections (oral cavity)
    Specification
    oral cavity
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 05 2013

    Western blot abreview for Anti-Twist antibody [Twist2C1a] - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (primary humanmacrophages)
    Specification
    primary humanmacrophages
    Treatment
    MDP 100ug/ml
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 24 2013

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Sample
    Human Cell lysate - whole cell (Different Cell types)
    Specification
    Different Cell types
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 10 2013

    1-10 of 43 Abreviews or Q&A

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