Recombinant Anti-Twist (phospho S68) antibody [EP18178] (ab187008)
![Western blot - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)](https://www.abcam.com/ps/products/187/ab187008/Images/ab187008-279232-anti-twist-phospho-s68-antibody-ep18178-western-blot.jpg "Western blot - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP18178] to Twist (phospho S68)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Twist (phospho S68) antibody [EP18178]
See all Twist primary antibodies -
Description
Rabbit monoclonal [EP18178] to Twist (phospho S68) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate. IP: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP18178 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 21 kDa).
|
| IP |
1/60.
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| Dot blot |
1/1000.
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 21 kDa). |
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IP
1/60. |
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Dot blot
1/1000. |
Target
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Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. -
Tissue specificity
Subset of mesodermal cells. -
Involvement in disease
Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7291 Human
- Omim: 601622 Human
- SwissProt: Q15672 Human
- Unigene: 66744 Human
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Alternative names
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
see all
Images
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Western blot - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)All lanes : Anti-Twist (phospho S68) antibody [EP18178] (ab187008) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
PMID: 21502402.
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![Dot Blot - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)](https://www.abcam.com/ps/products/187/ab187008/Images/ab187008-279233-anti-twist-phospho-s68-antibody-ep18178-dot-blot.jpg)
Dot Blot - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)Dot blot analysis of Twist (phospho S68) labeled with ab187008 at 1/1000 dilution.
Lane 1: Twist (phospho S68) peptide;
Lane 2: Twist non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)](https://www.abcam.com/ps/products/187/ab187008/Images/ab187008-279231-anti-twist-phospho-s68-antibody-ep18178-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)Twist (phospho S68) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate with ab187008 at 1/60 dilution.
Western blot was performed from the immunoprecipitate using ab187008 at 1/5000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate 10ug (Input).
Lane 2: ab187008 IP in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187008 in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab187008 has been referenced in 7 publications.
- Fan J et al. MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation. Cell Death Discov 8:2 (2022). WB ; Rat . PubMed: 35013126
- Cui F et al. Silencing of Long Non-Coding RNA FGD5-AS1 Inhibits the Progression of Non-Small Cell Lung Cancer by Regulating the miR-493-5p/DDX5 Axis. Technol Cancer Res Treat 20:1533033821990007 (2021). PubMed: 33550957
- Shen H et al. ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression. Cell Death Dis 12:349 (2021). PubMed: 33824282
- Wang SC et al. a-Linolenic acid inhibits the migration of human triple-negative breast cancer cells by attenuating Twist1 expression and suppressing Twist1-mediated epithelial-mesenchymal transition. Biochem Pharmacol 180:114152 (2020). WB ; Human . PubMed: 32679125
- Xu J et al. LncRNA BBOX1-AS1 upregulates HOXC6 expression through miR-361-3p and HuR to drive cervical cancer progression. Cell Prolif 53:e12823 (2020). PubMed: 32515533
- Guo L et al. OIP5-AS1/miR-137/ZNF217 Axis Promotes Malignant Behaviors in Epithelial Ovarian Cancer. Cancer Manag Res 12:6707-6717 (2020). PubMed: 32801903
- Cheng L et al. GBP1 promotes erlotinib resistance via PGK1-activated EMT signaling in non-small cell lung cancer. Int J Oncol 57:858-870 (2020). PubMed: 32582960